Detailed information of TA system TA109759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5090735..5091416
• Replicon: chromosome
• Accession: NZ_CP031677
• Organism: Pseudomonas aeruginosa strain E80

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DOM02_RS24605
• Protein ID: WP_015503432.1
• Coordinates: 5091051..5091416 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DOM02_RS24600
• Protein ID: WP_016561576.1
• Coordinates: 5090735..5091058 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOM02_RS24560	5086355..5087233	+	879	WP_003109435.1	hypothetical protein	-
DOM02_RS24570	5087745..5088611	-	867	WP_023081971.1	hypothetical protein	-
DOM02_RS24575	5088641..5088892	-	252	WP_124136459.1	hypothetical protein	-
DOM02_RS24580	5089109..5089663	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
DOM02_RS24585	5089838..5090113	-	276	WP_004353146.1	hypothetical protein	-
DOM02_RS24590	5090113..5090445	-	333	WP_016561578.1	hypothetical protein	-
DOM02_RS24595	5090500..5090619	+	120	Protein_4726	IS5/IS1182 family transposase	-
DOM02_RS24600	5090735..5091058	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
DOM02_RS24605	5091051..5091416	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DOM02_RS24610	5091680..5091922	-	243	WP_043884955.1	hypothetical protein	-
DOM02_RS24615	5092129..5092401	+	273	WP_003085667.1	hypothetical protein	-
DOM02_RS24620	5092420..5092845	-	426	WP_003163196.1	VOC family protein	-
DOM02_RS24625	5092946..5093830	+	885	WP_019725847.1	LysR family transcriptional regulator	-
DOM02_RS24630	5093803..5094756	-	954	WP_003085661.1	LysR family transcriptional regulator	-
DOM02_RS24635	5094977..5095411	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T109759 WP_015503432.1 NZ_CP031677:c5091416-5091051 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T109759 NZ_CP031677:c5091416-5091051 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT109759 WP_016561576.1 NZ_CP031677:c5091058-5090735 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT109759 NZ_CP031677:c5091058-5090735 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA