Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2325276..2325778 Replicon chromosome
Accession NZ_CP031677
Organism Pseudomonas aeruginosa strain E80

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1C7CBF7
Locus tag DOM02_RS10870 Protein ID WP_023081358.1
Coordinates 2325276..2325530 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A1C7C600
Locus tag DOM02_RS10875 Protein ID WP_004350479.1
Coordinates 2325527..2325778 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DOM02_RS10850 2321711..2322574 + 864 WP_004350470.1 integrase domain-containing protein -
DOM02_RS10855 2322762..2323112 + 351 WP_023081356.1 hypothetical protein -
DOM02_RS10860 2323127..2324647 + 1521 WP_004350477.1 conjugal transfer protein TraG N-terminal domain-containing protein -
DOM02_RS10865 2324655..2325050 - 396 WP_023081357.1 DUF3742 family protein -
DOM02_RS10870 2325276..2325530 - 255 WP_023081358.1 Txe/YoeB family addiction module toxin Toxin
DOM02_RS10875 2325527..2325778 - 252 WP_004350479.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DOM02_RS10880 2325863..2327233 - 1371 WP_023081359.1 tyrosine-type recombinase/integrase -
DOM02_RS10890 2327601..2328161 - 561 WP_003090349.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
DOM02_RS10895 2328195..2330021 - 1827 WP_003097551.1 excinuclease ABC subunit UvrC -
DOM02_RS10900 2330022..2330666 - 645 WP_003090351.1 UvrY/SirA/GacA family response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2316553..2325778 9225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10061.50 Da        Isoelectric Point: 8.4789

>T109755 WP_023081358.1 NZ_CP031677:c2325530-2325276 [Pseudomonas aeruginosa]
VKLIWSEQAWDDYLYWQDTDKRMVKKVNELIKDACRTPFEGKGKPEPLRHNLAGFWSRRITAEHRFVYSVTDDALLIAAC
RYHY

Download         Length: 255 bp

>T109755 NZ_CP031677:c2325530-2325276 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9363.65 Da        Isoelectric Point: 4.5843

>AT109755 WP_004350479.1 NZ_CP031677:c2325778-2325527 [Pseudomonas aeruginosa]
MRTISYTEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEFNALEETAYLLRSPANARRLMDSIDSLKQGQGVERD
LAE

Download         Length: 252 bp

>AT109755 NZ_CP031677:c2325778-2325527 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C7CBF7

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C600

References