Detailed information of TA system TA109659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5475994..5476589
• Replicon: chromosome
• Accession: NZ_CP031660
• Organism: Pseudomonas aeruginosa strain PABL017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: DZ899_RS26095
• Protein ID: WP_003113526.1
• Coordinates: 5476311..5476589 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DZ899_RS26090
• Protein ID: WP_003099268.1
• Coordinates: 5475994..5476299 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ899_RS26055	5471133..5471981	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
DZ899_RS26065	5472148..5473089	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
DZ899_RS26070	5473206..5473820	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
DZ899_RS26075	5473862..5474446	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
DZ899_RS26080	5474487..5475587	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
DZ899_RS26090	5475994..5476299	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
DZ899_RS26095	5476311..5476589	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZ899_RS26105	5476918..5479146	+	2229	WP_003113525.1	TonB-dependent receptor	-
DZ899_RS26110	5479216..5479863	-	648	WP_003095021.1	carbonate dehydratase	-
DZ899_RS26115	5479925..5481163	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T109659 WP_003113526.1 NZ_CP031660:c5476589-5476311 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T109659 NZ_CP031660:c5476589-5476311 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT109659 WP_003099268.1 NZ_CP031660:c5476299-5475994 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT109659 NZ_CP031660:c5476299-5475994 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3