109658

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4769939..4770620 Replicon chromosome
Accession NZ_CP031660
Organism Pseudomonas aeruginosa strain PABL017

Toxin (Protein)


Gene name higB Uniprot ID V6AKP0
Locus tag DZ899_RS22740 Protein ID WP_003111825.1
Coordinates 4770255..4770620 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1C7BMJ2
Locus tag DZ899_RS22735 Protein ID WP_003159602.1
Coordinates 4769939..4770262 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DZ899_RS22695 4765203..4765436 - 234 WP_117341318.1 AlpA family transcriptional regulator -
DZ899_RS22700 4765506..4766420 - 915 WP_205521068.1 hypothetical protein -
DZ899_RS22705 4767042..4767716 + 675 WP_117341320.1 hypothetical protein -
DZ899_RS22710 4768083..4768310 + 228 WP_014603215.1 hypothetical protein -
DZ899_RS22715 4768325..4768867 - 543 WP_019396018.1 tyrosine-type recombinase/integrase -
DZ899_RS22720 4769042..4769317 - 276 WP_004353146.1 hypothetical protein -
DZ899_RS22725 4769317..4769649 - 333 WP_021205399.1 hypothetical protein -
DZ899_RS22730 4769704..4769823 + 120 Protein_4381 IS5/IS1182 family transposase -
DZ899_RS22735 4769939..4770262 - 324 WP_003159602.1 XRE family transcriptional regulator Antitoxin
DZ899_RS22740 4770255..4770620 - 366 WP_003111825.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DZ899_RS22745 4770913..4771173 - 261 WP_079386856.1 hypothetical protein -
DZ899_RS22750 4771380..4771652 + 273 WP_033942888.1 hypothetical protein -
DZ899_RS22755 4771683..4772108 - 426 WP_033942887.1 ring-cleaving dioxygenase -
DZ899_RS22760 4772209..4773093 + 885 WP_003111828.1 LysR family transcriptional regulator -
DZ899_RS22765 4773066..4774019 - 954 WP_024918262.1 LysR family transcriptional regulator -
DZ899_RS22770 4774240..4774674 + 435 WP_003116494.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4744269..4773093 28824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-63)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13880.24 Da        Isoelectric Point: 4.8219

>T109658 WP_003111825.1 NZ_CP031660:c4770620-4770255 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Download         Length: 366 bp

>T109658 NZ_CP031660:c4770620-4770255 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11882.71 Da        Isoelectric Point: 6.4751

>AT109658 WP_003159602.1 NZ_CP031660:c4770262-4769939 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT109658 NZ_CP031660:c4770262-4769939 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAACTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6AKP0

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7BMJ2

References