Detailed information of TA system TA109614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 149381..149883
• Replicon: plasmid unnamed2
• Accession: NZ_CP031651
• Organism: Pantoea agglomerans strain TH81

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8X8DPE2
• Locus tag: D0A61_RS22540
• Protein ID: WP_010256862.1
• Coordinates: 149629..149883 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A349IE86
• Locus tag: D0A61_RS22535
• Protein ID: WP_010256861.1
• Coordinates: 149381..149632 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0A61_RS22520	145083..146534	+	1452	WP_122460000.1	flagellin	-
D0A61_RS22525	146876..148189	+	1314	WP_010256858.1	CitMHS family transporter	-
D0A61_RS22530	148357..149196	+	840	WP_010256859.1	MBL fold metallo-hydrolase	-
D0A61_RS22535	149381..149632	+	252	WP_010256861.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D0A61_RS22540	149629..149883	+	255	WP_010256862.1	Txe/YoeB family addiction module toxin	Toxin
D0A61_RS22545	149939..152485	-	2547	WP_122460001.1	excinuclease ABC subunit UvrA	-
D0A61_RS22550	153145..153603	+	459	WP_033786449.1	hypothetical protein	-
D0A61_RS22555	153597..153890	+	294	WP_033786403.1	DUF1493 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..182169	182169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9782.16 Da
• Isoelectric Point: 9.3799

>T109614 WP_010256862.1 NZ_CP031651:149629-149883 [Pantoea agglomerans]
VKLTWSAEAWEDYLYWQDADKKTVKKINELLKDASRTPFEGKGKPEPLKHNLAGFWSRRITAEHRLVYAVSGDALLIASC
RYHY

Nucleotide

• Length: 255 bp

>T109614 NZ_CP031651:149629-149883 [Pantoea agglomerans]
GTGAAACTAACCTGGTCCGCAGAAGCCTGGGAAGATTATCTCTACTGGCAGGATGCCGATAAAAAAACCGTTAAAAAGAT
AAACGAGCTGCTCAAAGATGCCAGCAGGACGCCTTTTGAAGGCAAAGGAAAACCCGAGCCGTTAAAGCATAATCTTGCAG
GTTTCTGGTCACGCAGAATCACGGCAGAACATCGGCTGGTGTACGCTGTCAGCGGGGATGCTCTATTGATTGCCTCATGC
CGTTATCATTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.48 Da
• Isoelectric Point: 4.4155

>AT109614 WP_010256861.1 NZ_CP031651:149381-149632 [Pantoea agglomerans]
MRAISYSEARQNLSATMVQTVEDKAPVLITRQNGESCVLMSLEEYNSLEETAYLLRSPKNARRLMDSIESLQSGNGTDRD
IIE

Nucleotide

• Length: 252 bp

>AT109614 NZ_CP031651:149381-149632 [Pantoea agglomerans]
ATGAGAGCAATCAGTTACAGCGAAGCCCGACAGAATCTGTCTGCCACTATGGTTCAGACAGTTGAAGATAAAGCGCCGGT
ATTGATCACTCGCCAGAACGGTGAATCCTGCGTGCTGATGTCGCTTGAGGAATATAACTCACTGGAAGAGACGGCATACC
TGCTGCGGTCACCTAAAAATGCGCGCAGGTTGATGGATTCTATCGAAAGCCTGCAAAGCGGTAACGGGACTGACAGGGAT
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A349IE86