Detailed information of TA system TA109611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 328525..329346
• Replicon: plasmid unnamed1
• Accession: NZ_CP031650
• Organism: Pantoea agglomerans strain TH81

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D0A61_RS20900
• Protein ID: WP_122459846.1
• Coordinates: 328525..328995 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: D0A61_RS20905
• Protein ID: WP_033773852.1
• Coordinates: 329002..329346 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0A61_RS20890	323850..326252	+	2403	WP_122459844.1	maltodextrin phosphorylase	-
D0A61_RS20895	326261..328336	+	2076	WP_122459845.1	4-alpha-glucanotransferase	-
D0A61_RS20900	328525..328995	-	471	WP_122459846.1	type II toxin-antitoxin system YhaV family toxin	Toxin
D0A61_RS20905	329002..329346	-	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
D0A61_RS20910	329446..329661	-	216	WP_010247349.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D0A61_RS20915	329850..330788	-	939	WP_122459847.1	maltose operon protein MalM	-
D0A61_RS20920	330891..332192	-	1302	WP_122459848.1	maltoporin	-
D0A61_RS20925	332236..333345	-	1110	WP_122459849.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..520959	520959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18259.75 Da
• Isoelectric Point: 8.4646

>T109611 WP_122459846.1 NZ_CP031650:c328995-328525 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKHAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQDWANTNS

Nucleotide

• Length: 471 bp

>T109611 NZ_CP031650:c328995-328525 [Pantoea agglomerans]
ATGGAAGACAGCACGGTTATAAATGGATGGCACGTTTATGTTCATCCCTGTTTTGAATCGCAGCTGATTGCGCTGACCAA
TGAAGTGGAGTTACTCAGGGCTAAATATCCTGAAAGTTACCAGCGAAAAGCGACGACTAAGCTTTTAGCCGCTGTGTTTA
AAGTTATCAATAGTGAGATTTGCGCCGATCCACATCAGGCAAAGTTTCGCCAGGGCGATACGCTGGGAGAACAAAATAAG
CATTGGTTCCGTGCAAAGTTTTTGATGCAGTATCGTTTGTTCTTTAGATTCAGCGAGCAACATAAAACCATCATCCTTGC
ATGGATGAATGACGCTGAGACCAAACATGCTTATGGCAGTAAGCGTGACGCCTATAAAGTATTTTCAGGAATGTTGCATA
ACGGCTATCCACCTGACGACTGGGCATTGCTGTTAAAGCAATCTCAGGATTGGGCGAATACAAATTCTTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT109611 WP_033773852.1 NZ_CP031650:c329346-329002 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT109611 NZ_CP031650:c329346-329002 [Pantoea agglomerans]
ATGGGAACTCTGACTCATGCAAACATCTCTCTGCGTGCTTCATCAAAACTGACAGCGCGCAGCCAGACCACTATCCCTGC
CGCAGTTCGTGATGCGATGAATCTGCAACCTGGTGAGAATATCGAATACGCTGTTCTGCCGGGCGGCAAAGTCCTTATTA
CCCGTCAGGAAACGGTGACTGATGATGATCCAGTGATGGCGAGCTTTCTGAGTTTTCTTGCTGCTGACATACGCAACACA
CCTGCAAATATTAAAACGCTGGATCGAGGATTATTAACTCGCATTACATCGTTGACCGAAGGTATGGACGTTGATTTGGA
TGCTCCACTCATGGACGATGAGTAA