Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6450988..6451580 Replicon chromosome
Accession NZ_CP031648
Organism Pseudomonas fluorescens strain Pf275

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag DZG01_RS29795 Protein ID WP_116834129.1
Coordinates 6451302..6451580 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag DZG01_RS29790 Protein ID WP_116834128.1
Coordinates 6450988..6451293 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DZG01_RS29770 6446109..6447008 + 900 WP_109753435.1 proline iminopeptidase-family hydrolase -
DZG01_RS29775 6447508..6448989 + 1482 WP_116834125.1 leucine-rich repeat domain-containing protein -
DZG01_RS29780 6449090..6449923 + 834 WP_116834126.1 oxidoreductase -
DZG01_RS29785 6449946..6450881 + 936 WP_116834127.1 AraC family transcriptional regulator -
DZG01_RS29790 6450988..6451293 - 306 WP_116834128.1 HigA family addiction module antidote protein Antitoxin
DZG01_RS29795 6451302..6451580 - 279 WP_116834129.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DZG01_RS29800 6451823..6452911 - 1089 WP_116834130.1 GlxA family transcriptional regulator -
DZG01_RS29805 6453010..6453879 + 870 WP_116834131.1 ABC transporter substrate-binding protein -
DZG01_RS29810 6453982..6454746 + 765 WP_162893937.1 amino acid ABC transporter ATP-binding protein -
DZG01_RS29815 6454746..6455402 + 657 WP_109753442.1 amino acid ABC transporter permease -
DZG01_RS29820 6455399..6456082 + 684 WP_109753443.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10487.89 Da        Isoelectric Point: 7.8577

>T109602 WP_116834129.1 NZ_CP031648:c6451580-6451302 [Pseudomonas fluorescens]
MIRSFRCADTQVLFETGKTRRWAAILSVAERKLGILDAAVNLNDLRSPPGNRLEQLYGDREGQHSIRINGQYRICFVWGA
NGPENVEIVDYH

Download         Length: 279 bp

>T109602 NZ_CP031648:c6451580-6451302 [Pseudomonas fluorescens]
ATGATCAGAAGCTTCCGATGCGCCGACACCCAAGTGCTTTTTGAAACCGGCAAGACTCGGCGCTGGGCCGCCATTCTTTC
AGTGGCGGAACGAAAGCTGGGCATACTCGATGCGGCGGTCAACCTCAACGATCTGCGCTCCCCGCCTGGAAACAGGCTGG
AACAGTTGTATGGCGACAGGGAAGGCCAACACAGCATCCGGATTAACGGTCAGTACCGCATCTGTTTTGTCTGGGGCGCC
AACGGCCCCGAAAATGTAGAAATCGTAGATTATCATTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11131.89 Da        Isoelectric Point: 6.8700

>AT109602 WP_116834128.1 NZ_CP031648:c6451293-6450988 [Pseudomonas fluorescens]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSVSADVALRLAAALDTSAQFWLNLQTAYDLR
TAEIAKGEQIHKEVERIPHCA

Download         Length: 306 bp

>AT109602 NZ_CP031648:c6451293-6450988 [Pseudomonas fluorescens]
ATGTTTAAGAATGGCATGAGGCCCGTACACCCTGGAGAAATTCTCAAGGAAGAGTATCTGGAACCGCTTGGCCTTACGGC
CGCGGCGCTCGCGAGGGCTTTGCACGTGTCTGCCCCCACCGTAAATGACATCGTATTGATGCGCCGTAGCGTCAGTGCGG
ATGTGGCCTTGCGCCTGGCAGCAGCACTGGACACGTCAGCGCAGTTTTGGCTGAACTTGCAGACCGCGTATGATTTACGT
ACAGCAGAGATTGCGAAAGGGGAACAGATCCATAAGGAAGTGGAAAGAATTCCTCACTGCGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References