Detailed information of TA system TA109600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4928526..4929198
• Replicon: chromosome
• Accession: NZ_CP031648
• Organism: Pseudomonas fluorescens strain Pf275

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DZG01_RS22890
• Protein ID: WP_109754987.1
• Coordinates: 4928833..4929198 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DZG01_RS22885
• Protein ID: WP_109754986.1
• Coordinates: 4928526..4928840 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZG01_RS22875	4925681..4928005	+	2325	WP_116834287.1	hypothetical protein	-
DZG01_RS22880	4928042..4928503	+	462	WP_018607342.1	Lrp/AsnC family transcriptional regulator	-
DZG01_RS22885	4928526..4928840	-	315	WP_109754986.1	XRE family transcriptional regulator	Antitoxin
DZG01_RS22890	4928833..4929198	-	366	WP_109754987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZG01_RS22895	4929559..4931241	+	1683	WP_109754988.1	NAD(P)/FAD-dependent oxidoreductase	-
DZG01_RS22900	4931254..4932048	+	795	WP_109754989.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14013.68 Da
• Isoelectric Point: 4.9669

>T109600 WP_109754987.1 NZ_CP031648:c4929198-4928833 [Pseudomonas fluorescens]
MKWDVEYTDEFEMWWSCLSETEQDSVQASVMLLGDAGPHLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQDRWYQECVPLAERLYDEHLEALKREGFDNG

Nucleotide

• Length: 366 bp

>T109600 NZ_CP031648:c4929198-4928833 [Pseudomonas fluorescens]
ATGAAATGGGACGTTGAATACACCGACGAATTTGAGATGTGGTGGAGCTGTCTCAGTGAGACCGAGCAAGATTCTGTGCA
GGCCTCGGTGATGCTGCTTGGCGATGCCGGGCCGCATCTGGGTTTTCCTCACACCAGTGATATCAAGGGCTCGCGTCATG
GCAACCTTCGTGAGTTACGAGTACAACACGCAGGTAGGCCTTATCGTGTGCTCTATGCGTTTGATCCACGCCGTTGCGCC
ATCCTGCTGATCGGGGGAGACAAAACCGGCCAGGACCGTTGGTATCAGGAATGTGTGCCTTTGGCTGAACGTCTCTATGA
CGAGCATCTGGAAGCATTGAAGAGAGAGGGCTTTGACAATGGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12002.75 Da
• Isoelectric Point: 9.9863

>AT109600 WP_109754986.1 NZ_CP031648:c4928840-4928526 [Pseudomonas fluorescens]
MAKKFSDLIARRSPDSRARAETLYQKLRAEMPLHELRQAQELSQDALAKTLHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFAHQTQ

Nucleotide

• Length: 315 bp

>AT109600 NZ_CP031648:c4928840-4928526 [Pseudomonas fluorescens]
ATGGCTAAGAAATTTTCCGACTTGATTGCACGTCGTTCGCCTGACTCCCGGGCTCGTGCAGAGACTCTGTATCAGAAACT
GCGTGCAGAAATGCCGTTGCACGAGTTGCGTCAGGCGCAGGAGCTGAGCCAGGATGCTTTGGCCAAGACGCTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAACGCCGTACCGACATGTACATCAGTACGCTACGAAATTACATCCGCGCCATGGGG
GGGGAATTGGAGATTATCGCGACCTTCCCGGAGGGGCAGGTCAGGATCGAAAACTTCGCCCATCAAACACAGTAA