Detailed information of TA system TA109598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1093953..1094557
• Replicon: chromosome
• Accession: NZ_CP031648
• Organism: Pseudomonas fluorescens strain Pf275

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DZG01_RS05165
• Protein ID: WP_116831887.1
• Coordinates: 1094243..1094557 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N1CCA5
• Locus tag: DZG01_RS05160
• Protein ID: WP_058545613.1
• Coordinates: 1093953..1094240 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZG01_RS05135	1090011..1091060	+	1050	WP_116831885.1	NAD(P)-dependent alcohol dehydrogenase	-
DZG01_RS05140	1091186..1092403	+	1218	WP_116831886.1	MFS transporter	-
DZG01_RS05145	1092415..1092822	-	408	WP_109751616.1	DoxX family protein	-
DZG01_RS05150	1092910..1093308	+	399	WP_205419933.1	winged helix-turn-helix transcriptional regulator	-
DZG01_RS05155	1093324..1093809	-	486	WP_109751617.1	hypothetical protein	-
DZG01_RS05160	1093953..1094240	-	288	WP_058545613.1	helix-turn-helix domain-containing protein	Antitoxin
DZG01_RS05165	1094243..1094557	-	315	WP_116831887.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZG01_RS05170	1094688..1095119	+	432	WP_116831888.1	GNAT family N-acetyltransferase	-
DZG01_RS05175	1095116..1096474	-	1359	WP_109751620.1	chromate efflux transporter	-
DZG01_RS05180	1096653..1097312	+	660	WP_116831889.1	ribonuclease T2	-
DZG01_RS05185	1097524..1099095	+	1572	WP_116831890.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11934.03 Da
• Isoelectric Point: 10.1359

>T109598 WP_116831887.1 NZ_CP031648:c1094557-1094243 [Pseudomonas fluorescens]
MIFIETPIFTRRVKELMDDDDFAVLQKILVCNPSAGDVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKLIVEHWR

Nucleotide

• Length: 315 bp

>T109598 NZ_CP031648:c1094557-1094243 [Pseudomonas fluorescens]
ATGATTTTTATTGAAACTCCAATTTTTACCCGTCGGGTCAAAGAACTGATGGATGACGATGATTTCGCTGTCCTACAGAA
AATACTGGTCTGCAATCCTTCCGCTGGTGATGTCATGGAAGCCACCGGAGGGATCCGAAAGATTCGTATCGCAGCCAAAG
GCCATGGCAAACGGGGTGGGGCCAGGGTGATCTACTACCACTTCGTCCATGCTTCGCGGATCGCATTACTGATGATATAT
CCGAAAAATGAACAGCAAGACTTGACCGTCGATCAACGCAAAGCATTGAAATTGATTGTCGAGCACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.40 Da
• Isoelectric Point: 8.7885

>AT109598 WP_058545613.1 NZ_CP031648:c1094240-1093953 [Pseudomonas fluorescens]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Nucleotide

• Length: 288 bp

>AT109598 NZ_CP031648:c1094240-1093953 [Pseudomonas fluorescens]
ATGAGCAAATTTTTCGATGAGCTGATGGAAAGCGTACAACAGATGGATGAAATCGTCCGAGGCGAACGCCAGCCGTCACG
GGAGTTCATCGTCGATGCGTTGCAAGTGAAGGAAATACGCAAGGCTACCGGCCTGACCCAAGCCAAGTTCGCAGCACTGA
TCGATGTCCAACTGGGCACGCTTCGCAACTGGGAGCAGGGACGACGCGAGCCTACCGGCCCCGCTAAAGCACTGCTGCGG
GCGATTCATAATGATCCGAAACACGTGATCAGCGCCCTAGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6N1CCA5