Detailed information of TA system TA109564

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25833..26097
• Replicon: plasmid pZYST1C2
• Accession: NZ_CP031615
• Organism: Klebsiella pneumoniae strain ZYST1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: DWV03_RS27655
• Protein ID: WP_001303307.1
• Coordinates: 25945..26097 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	25833..25895 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWV03_RS27640	21935..23005	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
DWV03_RS27645	23024..24232	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	24412..24472	-	61	NuclAT_1	-	-
-	24412..24472	-	61	NuclAT_1	-	-
-	24412..24472	-	61	NuclAT_1	-	-
-	24412..24472	-	61	NuclAT_1	-	-
DWV03_RS27650	24539..25630	-	1092	WP_000426061.1	hypothetical protein	-
-	25833..25895	-	63	NuclAT_0	-	Antitoxin
-	25833..25895	-	63	NuclAT_0	-	Antitoxin
-	25833..25895	-	63	NuclAT_0	-	Antitoxin
-	25833..25895	-	63	NuclAT_0	-	Antitoxin
DWV03_RS27655	25945..26097	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
DWV03_RS29575	26462..26794	+	333	WP_162888547.1	hypothetical protein	-
-	26810..26862	-	53	NuclAT_2	-	-
-	26810..26862	-	53	NuclAT_2	-	-
-	26810..26862	-	53	NuclAT_2	-	-
-	26810..26862	-	53	NuclAT_2	-	-
DWV03_RS29580	27357..27533	-	177	WP_001054900.1	hypothetical protein	-
DWV03_RS27665	27742..27952	-	211	Protein_34	hemolysin expression modulator Hha	-
DWV03_RS27670	28050..28664	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
DWV03_RS27675	28740..30908	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / erm(B)	-	1..107458	107458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T109564 WP_001303307.1 NZ_CP031615:25945-26097 [Klebsiella pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T109564 NZ_CP031615:25945-26097 [Klebsiella pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT109564 NZ_CP031615:c25895-25833 [Klebsiella pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin