Detailed information of TA system TA109545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13554..14211
• Replicon: plasmid pIncAC2-1502318
• Accession: NZ_CP031610
• Organism: Escherichia coli strain N3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AXA56_RS24025
• Protein ID: WP_000270043.1
• Coordinates: 13861..14211 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA56_RS24020
• Protein ID: WP_000124640.1
• Coordinates: 13554..13856 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA56_RS23970	9189..9617	+	429	WP_000591076.1	hypothetical protein	-
AXA56_RS23975	9675..10034	+	360	WP_000422769.1	hypothetical protein	-
AXA56_RS23980	10034..10480	+	447	WP_000919343.1	hypothetical protein	-
AXA56_RS23985	10477..10995	+	519	WP_000210757.1	nitrite reductase	-
AXA56_RS23990	10995..11224	+	230	Protein_15	hypothetical protein	-
AXA56_RS23995	11211..12068	+	858	WP_001167036.1	hypothetical protein	-
AXA56_RS25260	11980..12285	+	306	WP_171820680.1	hypothetical protein	-
AXA56_RS24005	12288..12815	+	528	WP_004201083.1	thermonuclease family protein	-
AXA56_RS24010	12873..13145	+	273	WP_001043046.1	HU family DNA-binding protein	-
AXA56_RS24015	13234..13527	+	294	WP_001239998.1	hypothetical protein	-
AXA56_RS24020	13554..13856	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AXA56_RS24025	13861..14211	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA56_RS24030	14374..14922	+	549	WP_001061573.1	hypothetical protein	-
AXA56_RS24040	15263..15457	+	195	WP_000343597.1	hypothetical protein	-
AXA56_RS24045	15468..15839	+	372	WP_000516918.1	hypothetical protein	-
AXA56_RS24050	15832..16302	+	471	WP_001281821.1	hypothetical protein	-
AXA56_RS24055	16317..16652	-	336	WP_000683477.1	hypothetical protein	-
AXA56_RS24060	16749..17237	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AXA56_RS24065	17240..17737	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / qnrA1 / mph(A) / ant(3'')-Ia / dfrA1 / aac(6')-Il / blaVIM-1	-	1..160292	160292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T109545 WP_000270043.1 NZ_CP031610:c14211-13861 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T109545 NZ_CP031610:c14211-13861 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT109545 WP_000124640.1 NZ_CP031610:c13856-13554 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT109545 NZ_CP031610:c13856-13554 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9