Detailed information of TA system TA109544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4712332..4713026
• Replicon: chromosome
• Accession: NZ_CP031609
• Organism: Escherichia coli strain N3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: AXA56_RS23385
• Protein ID: WP_001263491.1
• Coordinates: 4712332..4712730 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: AXA56_RS23390
• Protein ID: WP_042003128.1
• Coordinates: 4712733..4713026 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA56_RS23355	4707569..4708027	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AXA56_RS23360	4708288..4709745	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AXA56_RS23365	4709802..4710416	-	615	WP_116836779.1	peptide chain release factor H	-
AXA56_RS23370	4710413..4711552	-	1140	WP_200834218.1	RNA ligase RtcB family protein	-
AXA56_RS23380	4711870..4712322	-	453	WP_072019097.1	GNAT family N-acetyltransferase	-
AXA56_RS23385	4712332..4712730	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AXA56_RS23390	4712733..4713026	-	294	WP_042003128.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AXA56_RS23395	4713078..4714133	-	1056	WP_001226166.1	DNA polymerase IV	-
AXA56_RS23400	4714204..4714989	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AXA56_RS23405	4714961..4716673	+	1713	Protein_4282	flagellar biosynthesis protein FlhA	-
AXA56_RS23410	4716835..4717584	-	750	WP_000093873.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T109544 WP_001263491.1 NZ_CP031609:c4712730-4712332 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T109544 NZ_CP031609:c4712730-4712332 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTAAATAATCCATTTCCGCCACAGTTGCGCCAATTTAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.82 Da
• Isoelectric Point: 5.9050

>AT109544 WP_042003128.1 NZ_CP031609:c4713026-4712733 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT109544 NZ_CP031609:c4713026-4712733 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7