Detailed information of TA system TA109497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13553..14210
• Replicon: plasmid pIncAC2-1502320
• Accession: NZ_CP031584
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AXA58_RS30125
• Protein ID: WP_000270043.1
• Coordinates: 13860..14210 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA58_RS30120
• Protein ID: WP_000124640.1
• Coordinates: 13553..13855 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS30070	9189..9617	+	429	WP_000591076.1	hypothetical protein	-
AXA58_RS30075	9675..10034	+	360	WP_000422769.1	hypothetical protein	-
AXA58_RS30080	10034..10479	+	446	Protein_13	Fe3+-siderophore ABC transporter permease	-
AXA58_RS30085	10476..10994	+	519	WP_000210757.1	nitrite reductase	-
AXA58_RS30090	10994..11224	+	231	WP_000972665.1	hypothetical protein	-
AXA58_RS30095	11211..12068	+	858	WP_001167036.1	hypothetical protein	-
AXA58_RS30105	12287..12814	+	528	WP_004201083.1	thermonuclease family protein	-
AXA58_RS30110	12872..13144	+	273	WP_001043046.1	HU family DNA-binding protein	-
AXA58_RS30115	13233..13526	+	294	WP_001239998.1	hypothetical protein	-
AXA58_RS30120	13553..13855	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AXA58_RS30125	13860..14210	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA58_RS30130	14373..14921	+	549	WP_001061573.1	hypothetical protein	-
AXA58_RS30140	15262..15456	+	195	WP_000343597.1	hypothetical protein	-
AXA58_RS30145	15467..15838	+	372	WP_000516918.1	hypothetical protein	-
AXA58_RS30150	15831..16301	+	471	WP_001281821.1	hypothetical protein	-
AXA58_RS30155	16316..16651	-	336	WP_000683477.1	hypothetical protein	-
AXA58_RS30160	16748..17236	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AXA58_RS30165	17239..17736	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / qacE / sul1 / qnrA1 / blaTEM-1B / mph(A) / ant(3'')-Ia / dfrA1 / aac(6')-Il / blaVIM-1	-	1..164082	164082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T109497 WP_000270043.1 NZ_CP031584:c14210-13860 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T109497 NZ_CP031584:c14210-13860 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT109497 WP_000124640.1 NZ_CP031584:c13855-13553 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT109497 NZ_CP031584:c13855-13553 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9