Detailed information of TA system TA109482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2777862..2778508
• Replicon: chromosome
• Accession: NZ_CP031577
• Organism: Klebsiella pneumoniae strain N4b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A483JFR4
• Locus tag: AXA58_RS13800
• Protein ID: WP_004188313.1
• Coordinates: 2777862..2778209 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA58_RS13805
• Protein ID: WP_040152325.1
• Coordinates: 2778209..2778508 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA58_RS13790	2773788..2775221	+	1434	WP_002920564.1	glycogen synthase GlgA	-
AXA58_RS13795	2775239..2777686	+	2448	WP_002920561.1	glycogen phosphorylase	-
AXA58_RS13800	2777862..2778209	+	348	WP_004188313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA58_RS13805	2778209..2778508	+	300	WP_040152325.1	helix-turn-helix domain-containing protein	Antitoxin
AXA58_RS13810	2778571..2780079	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
AXA58_RS13815	2780284..2780613	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AXA58_RS13820	2780664..2781494	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
AXA58_RS13825	2781544..2782302	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13548.57 Da
• Isoelectric Point: 6.2327

>T109482 WP_004188313.1 NZ_CP031577:2777862-2778209 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T109482 NZ_CP031577:2777862-2778209 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10830.61 Da
• Isoelectric Point: 10.1464

>AT109482 WP_040152325.1 NZ_CP031577:2778209-2778508 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT109482 NZ_CP031577:2778209-2778508 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A483JFR4