Detailed information of TA system TA109456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13138..13795
• Replicon: plasmid pIncAC2-1502262
• Accession: NZ_CP031573
• Organism: Enterobacter hormaechei strain N1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AXA51_RS24355
• Protein ID: WP_000270043.1
• Coordinates: 13445..13795 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA51_RS24350
• Protein ID: WP_000124640.1
• Coordinates: 13138..13440 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA51_RS24300	8772..9200	+	429	WP_000591076.1	hypothetical protein	-
AXA51_RS24305	9258..9617	+	360	WP_000422769.1	hypothetical protein	-
AXA51_RS24310	9617..10063	+	447	WP_000919343.1	hypothetical protein	-
AXA51_RS24315	10060..10578	+	519	WP_000210757.1	nitrite reductase	-
AXA51_RS24320	10578..10808	+	231	WP_000972665.1	hypothetical protein	-
AXA51_RS24325	10795..11652	+	858	WP_001167036.1	hypothetical protein	-
AXA51_RS25500	11564..11869	+	306	WP_171820680.1	hypothetical protein	-
AXA51_RS24335	11872..12399	+	528	WP_004201083.1	thermonuclease family protein	-
AXA51_RS24340	12457..12729	+	273	WP_001043046.1	HU family DNA-binding protein	-
AXA51_RS24345	12818..13111	+	294	WP_001239998.1	hypothetical protein	-
AXA51_RS24350	13138..13440	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AXA51_RS24355	13445..13795	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA51_RS24360	13958..14506	+	549	WP_001061573.1	hypothetical protein	-
AXA51_RS24370	14847..15041	+	195	WP_000343597.1	hypothetical protein	-
AXA51_RS24375	15052..15423	+	372	WP_000516918.1	hypothetical protein	-
AXA51_RS24380	15416..15886	+	471	WP_001281821.1	hypothetical protein	-
AXA51_RS24385	15901..16236	-	336	WP_000683477.1	hypothetical protein	-
AXA51_RS24390	16333..16821	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AXA51_RS24395	16824..17321	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / qacE / sul1 / qnrA1 / blaTEM-1B / mph(A) / ant(3'')-Ia / dfrA1 / aac(6')-Il / blaVIM-1	-	1..164095	164095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T109456 WP_000270043.1 NZ_CP031573:c13795-13445 [Enterobacter hormaechei]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T109456 NZ_CP031573:c13795-13445 [Enterobacter hormaechei]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT109456 WP_000124640.1 NZ_CP031573:c13440-13138 [Enterobacter hormaechei]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT109456 NZ_CP031573:c13440-13138 [Enterobacter hormaechei]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9