Detailed information of TA system TA109442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1500262..1500922
• Replicon: chromosome
• Accession: NZ_CP031571
• Organism: Enterobacter hormaechei strain N1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AXA51_RS07310
• Protein ID: WP_110292920.1
• Coordinates: 1500262..1500615 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA51_RS07315
• Protein ID: WP_110292919.1
• Coordinates: 1500620..1500922 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA51_RS07300	1499086..1499547	+	462	WP_049004530.1	hypothetical protein	-
AXA51_RS07305	1499544..1499765	+	222	WP_047738806.1	helix-turn-helix domain-containing protein	-
AXA51_RS07310	1500262..1500615	+	354	WP_110292920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA51_RS07315	1500620..1500922	+	303	WP_110292919.1	XRE family transcriptional regulator	Antitoxin
AXA51_RS07325	1501427..1502314	+	888	WP_110292918.1	integrase domain-containing protein	-
AXA51_RS07330	1503012..1504274	-	1263	WP_129541326.1	integrase arm-type DNA-binding domain-containing protein	-
AXA51_RS07340	1504832..1505749	+	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1488965..1508129	19164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13444.27 Da
• Isoelectric Point: 8.9507

>T109442 WP_110292920.1 NZ_CP031571:1500262-1500615 [Enterobacter hormaechei]
VWTIKTTDTFDRWFASLNDTDRVSVLAALLVLREKGPGLSRPYADTVRGSHYSNMKELRIQSRGDPIRAFFAFDPTRTGI
VLCAGNKVGNEKRFYDEMLPVADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T109442 NZ_CP031571:1500262-1500615 [Enterobacter hormaechei]
GTGTGGACAATTAAAACAACGGATACGTTTGATCGCTGGTTTGCCTCGCTCAACGATACCGACAGGGTCAGCGTGCTTGC
AGCACTGCTGGTCCTGCGGGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGGTCAGGGGTTCCCATTACAGCA
ACATGAAAGAGCTGCGTATTCAGAGTCGGGGCGATCCGATAAGGGCGTTTTTCGCTTTTGACCCGACCCGTACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGACAGGGAATTCAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11099.88 Da
• Isoelectric Point: 6.4738

>AT109442 WP_110292919.1 NZ_CP031571:1500620-1500922 [Enterobacter hormaechei]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKRGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT109442 NZ_CP031571:1500620-1500922 [Enterobacter hormaechei]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAACATTCACCTTGCCGAACTGCGTGAGAAAGTACAGAAAACGCAGGTGGAGATGGCACAAGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACGAGGGCGCGATCTGAAGCTTTCCACTCTCAAACGCTACGTTGAAGCGACT
GGCGGTAAGCTACGTCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTTGTACTGTAA