Detailed information of TA system TA109438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13554..14211
• Replicon: plasmid pIncAC2-1301491
• Accession: NZ_CP031570
• Organism: Enterobacter hormaechei strain 2013_1a

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AXA59_RS26075
• Protein ID: WP_000270043.1
• Coordinates: 13861..14211 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXA59_RS26070
• Protein ID: WP_000124640.1
• Coordinates: 13554..13856 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA59_RS26020	9189..9617	+	429	WP_000591076.1	hypothetical protein	-
AXA59_RS26025	9675..10067	+	393	WP_129557096.1	hypothetical protein	-
AXA59_RS26030	10033..10479	+	447	WP_000919343.1	hypothetical protein	-
AXA59_RS26035	10476..10994	+	519	WP_000210757.1	nitrite reductase	-
AXA59_RS26040	10994..11224	+	231	WP_000972665.1	hypothetical protein	-
AXA59_RS26045	11211..12068	+	858	WP_001167036.1	hypothetical protein	-
AXA59_RS27260	11980..12285	+	306	WP_171820680.1	hypothetical protein	-
AXA59_RS26055	12288..12815	+	528	WP_004201083.1	thermonuclease family protein	-
AXA59_RS26060	12873..13145	+	273	WP_001043046.1	HU family DNA-binding protein	-
AXA59_RS26065	13234..13527	+	294	WP_001239998.1	hypothetical protein	-
AXA59_RS26070	13554..13856	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AXA59_RS26075	13861..14211	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA59_RS26080	14374..14922	+	549	WP_001061573.1	hypothetical protein	-
AXA59_RS26090	15263..15457	+	195	WP_000343597.1	hypothetical protein	-
AXA59_RS26095	15468..15839	+	372	WP_000516918.1	hypothetical protein	-
AXA59_RS26100	15832..16302	+	471	WP_001281821.1	hypothetical protein	-
AXA59_RS26105	16317..16652	-	336	WP_000683477.1	hypothetical protein	-
AXA59_RS26110	16749..17237	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AXA59_RS26115	17240..17737	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaVIM-1 / aac(6')-Il / dfrA1 / ant(3'')-Ia / mph(A) / sul1 / qnrA1 / qacE / aadA2 / dfrA12	-	1..158968	158968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T109438 WP_000270043.1 NZ_CP031570:c14211-13861 [Enterobacter hormaechei]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T109438 NZ_CP031570:c14211-13861 [Enterobacter hormaechei]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT109438 WP_000124640.1 NZ_CP031570:c13856-13554 [Enterobacter hormaechei]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT109438 NZ_CP031570:c13856-13554 [Enterobacter hormaechei]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9