Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 616269..616782 Replicon chromosome
Accession NZ_CP031556
Organism Streptococcus agalactiae strain Sag27

Toxin (Protein)


Gene name yoeB Uniprot ID Q8DX49
Locus tag DY328_RS03215 Protein ID WP_000481712.1
Coordinates 616269..616523 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q8DX48
Locus tag DY328_RS03220 Protein ID WP_000387490.1
Coordinates 616516..616782 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DY328_RS03195 612041..614542 - 2502 WP_000239405.1 ATP-binding protein -
DY328_RS03200 614563..614973 - 411 WP_000390748.1 conjugal transfer protein -
DY328_RS03205 614977..615243 - 267 WP_001055036.1 hypothetical protein -
DY328_RS03210 615263..616216 - 954 WP_000565542.1 conjugal transfer protein -
DY328_RS03215 616269..616523 - 255 WP_000481712.1 Txe/YoeB family addiction module toxin Toxin
DY328_RS03220 616516..616782 - 267 WP_000387490.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
DY328_RS03225 616927..617400 - 474 WP_000560386.1 hypothetical protein -
DY328_RS03230 617408..617890 - 483 WP_000383282.1 antirestriction protein ArdA -
DY328_RS03235 617942..618214 - 273 WP_000072642.1 hypothetical protein -
DY328_RS03240 618346..618699 - 354 WP_000571928.1 helix-turn-helix domain-containing protein -
DY328_RS03245 618692..618907 - 216 WP_001897988.1 helix-turn-helix domain-containing protein -
DY328_RS03250 618891..619268 - 378 WP_000287184.1 hypothetical protein -
DY328_RS03255 619284..620516 - 1233 WP_000338839.1 replication initiation factor domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 603074..641985 38911


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-83)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10445.87 Da        Isoelectric Point: 6.7282

>T109397 WP_000481712.1 NZ_CP031556:c616523-616269 [Streptococcus agalactiae]
MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKPEPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFR
DHYK

Download         Length: 255 bp

>T109397 NZ_CP031556:c616523-616269 [Streptococcus agalactiae]
ATGTTTAATTTTACTGAAGAAGCATGGAAAGATTATGTTTCTTGGCAACAGGAAGATAAAAAAATCTTGAAACGAATTAA
TCGTTTGATAGAAGATATTAAAAGAGACCCATTTGAAGGCATTGGAAAACCAGAACCTTTAAAATACCATTATTCAGGGG
CTTGGTCTCGAAGAATTACAGAAGAACATAGGTTGATTTATATGATAGAAGATGGAGAAATCTATTTTTTATCTTTTAGA
GATCATTATAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10316.70 Da        Isoelectric Point: 4.6742

>AT109397 WP_000387490.1 NZ_CP031556:c616782-616516 [Streptococcus agalactiae]
MEAIVYSHFRNNLKDYMKKVNDEFEPLIVVNKNPDENIVVLSQDSWESLQETIRLMENDYLSHKVINGISQVKEKQVTKH
GLIEVEDV

Download         Length: 267 bp

>AT109397 NZ_CP031556:c616782-616516 [Streptococcus agalactiae]
ATGGAGGCTATTGTTTATTCACATTTTAGAAATAATTTAAAAGATTATATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GATTGTAGTTAATAAAAATCCTGATGAAAATATAGTGGTTTTATCTCAAGATAGCTGGGAAAGTTTACAAGAAACGATTC
GTTTGATGGAAAATGATTATCTATCACATAAGGTTATTAATGGTATATCACAAGTTAAAGAGAAGCAAGTCACTAAACAT
GGTTTAATTGAGGTAGAAGATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IC12

Antitoxin

Source ID Structure
AlphaFold DB A0A829I9V6

References