Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 172184..172841 | Replicon | plasmid unnamed3 |
| Accession | NZ_CP031549 | ||
| Organism | Escherichia coli strain cq9 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | DS732_RS30850 | Protein ID | WP_000270043.1 |
| Coordinates | 172491..172841 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | DS732_RS30845 | Protein ID | WP_000124640.1 |
| Coordinates | 172184..172486 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DS732_RS30795 | 167809..168237 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| DS732_RS30800 | 168294..168653 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| DS732_RS30805 | 168653..169099 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| DS732_RS30810 | 169096..169614 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| DS732_RS30815 | 169614..169844 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| DS732_RS30820 | 169831..170688 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| DS732_RS30825 | 170714..170902 | + | 189 | WP_001270411.1 | hypothetical protein | - |
| DS732_RS30830 | 170919..171446 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| DS732_RS30835 | 171504..171776 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| DS732_RS30840 | 171864..172157 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| DS732_RS30845 | 172184..172486 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| DS732_RS30850 | 172491..172841 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| DS732_RS30855 | 173004..173552 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| DS732_RS30865 | 173893..174087 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| DS732_RS30870 | 174098..174469 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| DS732_RS30875 | 174462..174932 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| DS732_RS30880 | 174947..175282 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| DS732_RS30885 | 175379..175867 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| DS732_RS30890 | 175870..176367 | + | 498 | WP_000062185.1 | hypothetical protein | - |
| DS732_RS30895 | 176582..177286 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| DS732_RS30900 | 177351..177530 | - | 180 | Protein_210 | winged helix-turn-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / erm(B) / mph(A) / floR | - | 1..189020 | 189020 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T109385 WP_000270043.1 NZ_CP031549:c172841-172491 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T109385 NZ_CP031549:c172841-172491 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT109385 WP_000124640.1 NZ_CP031549:c172486-172184 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT109385 NZ_CP031549:c172486-172184 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|