Detailed information of TA system TA109385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 172184..172841
• Replicon: plasmid unnamed3
• Accession: NZ_CP031549
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: DS732_RS30850
• Protein ID: WP_000270043.1
• Coordinates: 172491..172841 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DS732_RS30845
• Protein ID: WP_000124640.1
• Coordinates: 172184..172486 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS30795	167809..168237	+	429	WP_000591074.1	hypothetical protein	-
DS732_RS30800	168294..168653	+	360	WP_000422768.1	hypothetical protein	-
DS732_RS30805	168653..169099	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
DS732_RS30810	169096..169614	+	519	WP_000210756.1	nitrite reductase	-
DS732_RS30815	169614..169844	+	231	WP_000972663.1	hypothetical protein	-
DS732_RS30820	169831..170688	+	858	WP_001167032.1	hypothetical protein	-
DS732_RS30825	170714..170902	+	189	WP_001270411.1	hypothetical protein	-
DS732_RS30830	170919..171446	+	528	WP_001236377.1	thermonuclease family protein	-
DS732_RS30835	171504..171776	+	273	WP_001043047.1	HU family DNA-binding protein	-
DS732_RS30840	171864..172157	+	294	WP_001239997.1	chromosome segregation protein ParM	-
DS732_RS30845	172184..172486	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
DS732_RS30850	172491..172841	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DS732_RS30855	173004..173552	+	549	WP_001061574.1	transcriptional regulator	-
DS732_RS30865	173893..174087	+	195	WP_000343597.1	hypothetical protein	-
DS732_RS30870	174098..174469	+	372	WP_000516916.1	hypothetical protein	-
DS732_RS30875	174462..174932	+	471	WP_001281821.1	hypothetical protein	-
DS732_RS30880	174947..175282	-	336	WP_000683476.1	hypothetical protein	-
DS732_RS30885	175379..175867	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
DS732_RS30890	175870..176367	+	498	WP_000062185.1	hypothetical protein	-
DS732_RS30895	176582..177286	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
DS732_RS30900	177351..177530	-	180	Protein_210	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / erm(B) / mph(A) / floR	-	1..189020	189020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T109385 WP_000270043.1 NZ_CP031549:c172841-172491 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T109385 NZ_CP031549:c172841-172491 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT109385 WP_000124640.1 NZ_CP031549:c172486-172184 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT109385 NZ_CP031549:c172486-172184 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9