Detailed information of TA system TA109380

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 200563..200984
• Replicon: plasmid unnamed2
• Accession: NZ_CP031548
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: DS732_RS29600
• Protein ID: WP_001302184.1
• Coordinates: 200563..200721 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	200786..200984 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS29565	195589..195954	-	366	WP_000338819.1	type IV conjugative transfer system pilin TraA	-
DS732_RS29570	195998..196213	-	216	WP_071782156.1	conjugal transfer relaxosome protein TraY	-
DS732_RS29575	196349..196996	-	648	WP_000332521.1	conjugal transfer transcriptional regulator TraJ	-
DS732_RS29580	197187..197570	-	384	WP_064986972.1	relaxosome protein TraM	-
DS732_RS29585	197892..198494	+	603	WP_077249085.1	transglycosylase SLT domain-containing protein	-
DS732_RS29590	198790..199611	-	822	WP_001424949.1	DUF945 domain-containing protein	-
DS732_RS29595	199729..200016	-	288	WP_000107541.1	hypothetical protein	-
DS732_RS32005	200076..200262	+	187	Protein_232	pilus protein	-
DS732_RS29600	200563..200721	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	200786..200984	-	199	NuclAT_0	-	Antitoxin
-	200786..200984	-	199	NuclAT_0	-	Antitoxin
-	200786..200984	-	199	NuclAT_0	-	Antitoxin
-	200786..200984	-	199	NuclAT_0	-	Antitoxin
DS732_RS29605	200996..201715	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
DS732_RS29610	201712..202146	-	435	WP_116794657.1	conjugation system SOS inhibitor PsiB	-
DS732_RS29615	202201..204159	-	1959	WP_000117204.1	ParB/RepB/Spo0J family partition protein	-
DS732_RS29620	204223..204456	-	234	WP_000005985.1	DUF905 family protein	-
DS732_RS29625	204518..205057	-	540	WP_000290811.1	single-stranded DNA-binding protein	-
DS732_RS32010	205083..205289	-	207	WP_000275848.1	hypothetical protein	-
DS732_RS29640	205359..205785	+	427	Protein_240	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / hlyC / hlyA / hlyB / hlyD / eltB / eltA	1..221041	221041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T109380 WP_001302184.1 NZ_CP031548:c200721-200563 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T109380 NZ_CP031548:c200721-200563 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT109380 NZ_CP031548:c200984-200786 [Escherichia coli]
TCATACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin