Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 4391455..4392148 Replicon chromosome
Accession NZ_CP031546
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag DS732_RS22650 Protein ID WP_000415584.1
Coordinates 4391852..4392148 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag DS732_RS22645 Protein ID WP_000650107.1
Coordinates 4391455..4391850 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS22630 4387319..4389577 - 2259 WP_064986897.1 DNA topoisomerase IV subunit A -
DS732_RS22635 4389715..4391322 - 1608 WP_001324265.1 ABC transporter substrate-binding protein -
DS732_RS22645 4391455..4391850 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
DS732_RS22650 4391852..4392148 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
DS732_RS22655 4392353..4392835 - 483 WP_000183494.1 transcriptional regulator -
DS732_RS22660 4392888..4393280 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
DS732_RS22665 4393432..4394091 + 660 WP_001221491.1 two-component system response regulator QseB -
DS732_RS22670 4394088..4395437 + 1350 WP_000673406.1 two-component system sensor histidine kinase QseC -
DS732_RS22675 4395547..4396128 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
DS732_RS22680 4396159..4396473 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rfaE 4387319..4460500 73181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T109370 WP_000415584.1 NZ_CP031546:c4392148-4391852 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T109370 NZ_CP031546:c4392148-4391852 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT109370 WP_000650107.1 NZ_CP031546:c4391850-4391455 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT109370 NZ_CP031546:c4391850-4391455 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References