Detailed information of TA system TA109366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3954635..3955362
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: DS732_RS20355
• Protein ID: WP_000547564.1
• Coordinates: 3955051..3955362 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DS732_RS20350
• Protein ID: WP_000126294.1
• Coordinates: 3954635..3955054 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS20330	3949685..3950212	-	528	WP_001078777.1	electron transport protein HydN	-
DS732_RS20335	3950361..3951371	-	1011	WP_063102913.1	DNA-binding transcriptional regulator AscG	-
DS732_RS20340	3951631..3953088	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
DS732_RS20345	3953097..3954521	+	1425	WP_063102937.1	6-phospho-beta-glucosidase AscB	-
DS732_RS20350	3954635..3955054	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
DS732_RS20355	3955051..3955362	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
DS732_RS20360	3955536..3956297	-	762	WP_001026446.1	hypothetical protein	-
DS732_RS20365	3956322..3956792	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
DS732_RS20370	3956785..3957195	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
DS732_RS20375	3957192..3957959	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
DS732_RS20380	3957959..3958501	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
DS732_RS20385	3958511..3960220	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T109366 WP_000547564.1 NZ_CP031546:c3955362-3955051 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T109366 NZ_CP031546:c3955362-3955051 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT109366 WP_000126294.1 NZ_CP031546:c3955054-3954635 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT109366 NZ_CP031546:c3955054-3954635 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5