109363

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3113076..3113578 Replicon chromosome
Accession NZ_CP031546
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DS732_RS16085 Protein ID WP_000767829.1
Coordinates 3113076..3113330 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DS732_RS16090 Protein ID WP_001259255.1
Coordinates 3113327..3113578 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS16055 3108091..3108318 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DS732_RS16060 3108332..3109390 - 1059 WP_023568741.1 transport protein YeeE -
DS732_RS16065 3109569..3110927 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DS732_RS16070 3110917..3110979 - 63 WP_010723108.1 membrane protein YoeI -
DS732_RS16075 3111194..3112123 - 930 WP_000803351.1 LysR family transcriptional regulator -
DS732_RS16080 3112169..3112993 - 825 WP_000754763.1 SDR family oxidoreductase -
DS732_RS16085 3113076..3113330 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DS732_RS16090 3113327..3113578 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DS732_RS16095 3113860..3113910 + 51 WP_001364200.1 his operon leader peptide -
DS732_RS16105 3114056..3114955 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DS732_RS16110 3114961..3116265 + 1305 WP_021823828.1 histidinol dehydrogenase -
DS732_RS16115 3116262..3117332 + 1071 WP_064986838.1 histidinol-phosphate transaminase -
DS732_RS16120 3117332..3118399 + 1068 WP_064986837.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109363 WP_000767829.1 NZ_CP031546:c3113330-3113076 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109363 NZ_CP031546:c3113330-3113076 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109363 WP_001259255.1 NZ_CP031546:c3113578-3113327 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109363 NZ_CP031546:c3113578-3113327 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References