Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3113076..3113578 Replicon chromosome
Accession NZ_CP031546
Organism Escherichia coli strain cq9

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DS732_RS16085 Protein ID WP_000767829.1
Coordinates 3113076..3113330 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DS732_RS16090 Protein ID WP_001259255.1
Coordinates 3113327..3113578 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DS732_RS16055 3108091..3108318 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DS732_RS16060 3108332..3109390 - 1059 WP_023568741.1 transport protein YeeE -
DS732_RS16065 3109569..3110927 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DS732_RS16070 3110917..3110979 - 63 WP_010723108.1 membrane protein YoeI -
DS732_RS16075 3111194..3112123 - 930 WP_000803351.1 LysR family transcriptional regulator -
DS732_RS16080 3112169..3112993 - 825 WP_000754763.1 SDR family oxidoreductase -
DS732_RS16085 3113076..3113330 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DS732_RS16090 3113327..3113578 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DS732_RS16095 3113860..3113910 + 51 WP_001364200.1 his operon leader peptide -
DS732_RS16105 3114056..3114955 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DS732_RS16110 3114961..3116265 + 1305 WP_021823828.1 histidinol dehydrogenase -
DS732_RS16115 3116262..3117332 + 1071 WP_064986838.1 histidinol-phosphate transaminase -
DS732_RS16120 3117332..3118399 + 1068 WP_064986837.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109363 WP_000767829.1 NZ_CP031546:c3113330-3113076 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109363 NZ_CP031546:c3113330-3113076 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109363 WP_001259255.1 NZ_CP031546:c3113578-3113327 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109363 NZ_CP031546:c3113578-3113327 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References