Detailed information of TA system TA109348

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2346082..2346453
• Replicon: chromosome
• Accession: NZ_CP031546
• Organism: Escherichia coli strain cq9

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: DS732_RS11925
• Protein ID: WP_021500490.1
• Coordinates: 2346259..2346453 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2346082..2346260 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DS732_RS11900	2342037..2343410	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
DS732_RS11905	2343539..2344474	-	936	WP_001774812.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DS732_RS11910	2344526..2345761	-	1236	WP_074149259.1	site-specific integrase	-
DS732_RS11915	2345763..2345978	-	216	WP_000079604.1	excisionase XisR	-
DS732_RS11920	2346078..2346266	-	189	WP_001383994.1	DUF1187 family protein	-
-	2346082..2346260	+	179	NuclAT_0	-	Antitoxin
-	2346082..2346260	+	179	NuclAT_0	-	Antitoxin
-	2346082..2346260	+	179	NuclAT_0	-	Antitoxin
-	2346082..2346260	+	179	NuclAT_0	-	Antitoxin
DS732_RS11925	2346259..2346453	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DS732_RS11930	2346517..2347569	-	1053	WP_001004422.1	recombinase RecT	-
DS732_RS11935	2347581..2350703	-	3123	WP_064986939.1	exodeoxyribonuclease VIII	-
DS732_RS11940	2350805..2351080	-	276	WP_000632298.1	hypothetical protein	-
DS732_RS11945	2351155..2351355	-	201	WP_071776730.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2302222..2392054	89832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T109348 WP_021500490.1 NZ_CP031546:c2346453-2346259 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T109348 NZ_CP031546:c2346453-2346259 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGCAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT109348 NZ_CP031546:2346082-2346260 [Escherichia coli]
GACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin