Detailed information of TA system TA109261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1212797..1213550
• Replicon: chromosome
• Accession: NZ_CP031467
• Organism: Paraburkholderia caffeinilytica strain CF1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: DSC91_RS21405
• Protein ID: WP_115780755.1
• Coordinates: 1212797..1213078 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DSC91_RS21410
• Protein ID: WP_115780756.1
• Coordinates: 1213095..1213550 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC91_RS21390	1208984..1209688	-	705	WP_115780752.1	DUF4136 domain-containing protein	-
DSC91_RS37575	1209687..1209875	+	189	WP_162831447.1	hypothetical protein	-
DSC91_RS21400	1209905..1212601	+	2697	WP_115780754.1	aminopeptidase N	-
DSC91_RS21405	1212797..1213078	+	282	WP_115780755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSC91_RS21410	1213095..1213550	+	456	WP_115780756.1	HigA family addiction module antidote protein	Antitoxin
DSC91_RS21415	1213673..1214689	+	1017	WP_115780757.1	class 1 fructose-bisphosphatase	-
DSC91_RS21425	1215008..1215208	-	201	WP_115780758.1	hypothetical protein	-
DSC91_RS21430	1215521..1216432	-	912	WP_175172033.1	LysR family transcriptional regulator	-
DSC91_RS21435	1216561..1217676	+	1116	WP_115780759.1	aldo/keto reductase	-
DSC91_RS37900	1218190..1218339	+	150	WP_167470519.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-116)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10521.20 Da
• Isoelectric Point: 10.3081

>T109261 WP_115780755.1 NZ_CP031467:1212797-1213078 [Paraburkholderia caffeinilytica]
MITTFGDKATAAIFQGKFVHSLPLHMQALARRKLLMIDAAASIRNLHAPPGNRLEALQGRRSGQWSIRVNAQWRICFYFV
DGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T109261 NZ_CP031467:1212797-1213078 [Paraburkholderia caffeinilytica]
ATGATCACGACATTCGGCGACAAGGCTACCGCGGCAATCTTTCAGGGCAAGTTCGTGCATTCACTGCCGCTTCACATGCA
GGCGCTCGCACGCCGTAAGCTGCTCATGATCGATGCAGCCGCGTCCATCCGCAACCTGCATGCGCCACCGGGCAACCGGC
TCGAAGCCTTGCAGGGCCGGCGCAGCGGGCAGTGGAGCATTCGCGTCAACGCGCAGTGGCGCATCTGTTTTTATTTTGTC
GACGGGGAGGCGTTGAATGTCGAAATTGTCGACTATCACTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16471.15 Da
• Isoelectric Point: 10.0448

>AT109261 WP_115780756.1 NZ_CP031467:1213095-1213550 [Paraburkholderia caffeinilytica]
MVIKRSDLGSIDFSDIATGDSIPEIHPGEILRCEFLEPLGMSVNALALALRVPAPRINDIVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKANRPKMPKIPAEHARAAALATHLAGHVTAIKARRKA

Nucleotide

• Length: 456 bp

>AT109261 NZ_CP031467:1213095-1213550 [Paraburkholderia caffeinilytica]
ATGGTCATCAAACGCTCCGATCTGGGCAGCATCGATTTCTCCGACATCGCCACCGGCGACAGCATCCCGGAAATCCATCC
CGGCGAGATTCTGCGGTGCGAGTTCCTCGAGCCGCTCGGTATGTCCGTCAATGCGCTCGCACTTGCGTTGCGCGTACCGG
CACCGCGCATCAATGACATCGTGCGCGGCAAACGCGCCATCTCGGCTGACACCGCGCTGCGGCTCGAACGCTATTTCGGC
GCGAGCGCGCAATTCTGGTTGAATCTGCAAATCGCCTATGATCTGCGCGTCGCCACCGCGGCGGCCGGCGAACAGATCGA
ACGCGAGATCGAGCCGATGCCCAAGGCGAACCGCCCCAAAATGCCGAAGATCCCGGCCGAACACGCCCGTGCCGCGGCGC
TTGCCACCCACCTGGCCGGCCATGTCACGGCCATCAAGGCTCGCCGCAAAGCCTGA