Detailed information of TA system TA109258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1873558..1874164
• Replicon: chromosome
• Accession: NZ_CP031466
• Organism: Paraburkholderia caffeinilytica strain CF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DSC91_RS08325
• Protein ID: WP_115777686.1
• Coordinates: 1873558..1873890 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3R7IKS7
• Locus tag: DSC91_RS08330
• Protein ID: WP_063498788.1
• Coordinates: 1873877..1874164 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC91_RS08310	1870149..1870949	+	801	WP_115777683.1	2-dehydro-3-deoxyglucarate aldolase	-
DSC91_RS08315	1870961..1871875	+	915	WP_115777684.1	2-hydroxy-3-oxopropionate reductase	-
DSC91_RS08320	1872017..1872838	-	822	WP_115777685.1	alpha/beta hydrolase	-
DSC91_RS08325	1873558..1873890	+	333	WP_115777686.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSC91_RS08330	1873877..1874164	+	288	WP_063498788.1	helix-turn-helix domain-containing protein	Antitoxin
DSC91_RS08335	1874495..1875907	+	1413	WP_115777687.1	cytochrome ubiquinol oxidase subunit I	-
DSC91_RS08340	1875932..1876936	+	1005	WP_115777688.1	cytochrome d ubiquinol oxidase subunit II	-
DSC91_RS08345	1876911..1877102	+	192	WP_115777689.1	hypothetical protein	-
DSC91_RS08350	1877443..1878201	+	759	WP_115777690.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12480.63 Da
• Isoelectric Point: 10.1359

>T109258 WP_115777686.1 NZ_CP031466:1873558-1873890 [Paraburkholderia caffeinilytica]
MAFTIDFYNEKVKVHVASLPKTLLARFIALADRMEEYGPNLGEPHTASMGGGLFEMRLKGAEGIARVFYCSIVERKIVML
HSFVKKSQQTPRNELDVARKRLLEIRNGNL

Nucleotide

• Length: 333 bp

>T109258 NZ_CP031466:1873558-1873890 [Paraburkholderia caffeinilytica]
ATGGCATTTACGATCGACTTCTACAACGAAAAGGTCAAAGTACACGTTGCCTCCTTGCCGAAAACGCTGCTTGCACGCTT
CATCGCGCTGGCCGATCGCATGGAGGAATACGGCCCGAATCTCGGCGAACCGCACACTGCATCAATGGGTGGCGGTTTAT
TCGAGATGCGCCTGAAAGGCGCCGAGGGCATCGCCAGAGTGTTCTACTGCTCCATCGTGGAACGGAAAATCGTCATGCTT
CACAGCTTTGTGAAGAAGTCGCAACAAACGCCGCGCAATGAACTGGACGTGGCGCGCAAAAGACTGCTGGAGATCAGAAA
TGGCAACTTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10594.17 Da
• Isoelectric Point: 9.6865

>AT109258 WP_063498788.1 NZ_CP031466:1873877-1874164 [Paraburkholderia caffeinilytica]
MATYKAMRERALADPEVRAEYERLNREEFALLDQMLAARREAGLTQAQVAERMGTKAPAITRLERALASGQHSPSIDTLR
RYAAACGKQLFISIA

Nucleotide

• Length: 288 bp

>AT109258 NZ_CP031466:1873877-1874164 [Paraburkholderia caffeinilytica]
ATGGCAACTTATAAGGCAATGCGCGAACGGGCGCTCGCCGACCCCGAAGTACGCGCCGAATACGAACGGCTGAACCGTGA
AGAATTCGCGCTGCTCGACCAGATGCTCGCCGCGCGCCGCGAAGCCGGCTTGACACAGGCGCAAGTCGCCGAGCGCATGG
GCACGAAAGCGCCGGCCATCACCCGGCTGGAGCGGGCACTTGCGTCGGGGCAGCACTCGCCTTCCATCGATACCTTACGC
AGATACGCGGCGGCGTGTGGCAAGCAGCTGTTTATCTCGATCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A3R7IKS7