Detailed information of TA system TA109256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4679656..4680245
• Replicon: chromosome
• Accession: NZ_CP031464
• Organism: Xanthomonas oryzae pv. oryzae strain YC11

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: BRN66_RS23225
• Protein ID: WP_011260747.1
• Coordinates: 4679964..4680245 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRN66_RS23220
• Protein ID: WP_011260746.1
• Coordinates: 4679656..4679946 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRN66_RS23180	4674935..4675408	-	474	Protein_4163	DUF4123 domain-containing protein	-
BRN66_RS23185	4675411..4675845	-	435	WP_011409747.1	PAAR domain-containing protein	-
BRN66_RS23190	4675961..4676206	-	246	WP_069960049.1	hypothetical protein	-
BRN66_RS23200	4676542..4676874	+	333	WP_011260743.1	hypothetical protein	-
BRN66_RS23210	4677324..4678718	-	1395	WP_113062041.1	nucleoside hydrolase	-
BRN66_RS23220	4679656..4679946	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRN66_RS23225	4679964..4680245	-	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRN66_RS23230	4680340..4682592	-	2253	WP_011409750.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.83 Da
• Isoelectric Point: 10.2139

>T109256 WP_011260747.1 NZ_CP031464:c4680245-4679964 [Xanthomonas oryzae pv. oryzae]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109256 NZ_CP031464:c4680245-4679964 [Xanthomonas oryzae pv. oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109256 WP_011260746.1 NZ_CP031464:c4679946-4679656 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109256 NZ_CP031464:c4679946-4679656 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA