Detailed information of TA system TA109249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 334129..334718
• Replicon: chromosome
• Accession: NZ_CP031463
• Organism: Xanthomonas oryzae pv. oryzae strain PX086

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: BRN52_RS01570
• Protein ID: WP_011260747.1
• Coordinates: 334129..334410 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRN52_RS01575
• Protein ID: WP_011260746.1
• Coordinates: 334428..334718 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRN52_RS01565	331782..334034	+	2253	WP_044756263.1	exodeoxyribonuclease V subunit alpha	-
BRN52_RS01570	334129..334410	+	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRN52_RS01575	334428..334718	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRN52_RS01585	335657..337051	+	1395	WP_011260745.1	nucleoside hydrolase	-
BRN52_RS01595	337501..337833	-	333	WP_011260743.1	hypothetical protein	-
BRN52_RS01605	338169..338393	+	225	WP_044756265.1	hypothetical protein	-
BRN52_RS01610	338531..338965	+	435	WP_011260742.1	PAAR domain-containing protein	-
BRN52_RS01615	338968..339444	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11038.77 Da
• Isoelectric Point: 10.2139

>T109249 WP_011260747.1 NZ_CP031463:334129-334410 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109249 NZ_CP031463:334129-334410 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109249 WP_011260746.1 NZ_CP031463:334428-334718 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109249 NZ_CP031463:334428-334718 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA