Detailed information of TA system TA109240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4760901..4761490
• Replicon: chromosome
• Accession: NZ_CP031460
• Organism: Xanthomonas oryzae pv. oryzae strain JP01

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: BRM88_RS23770
• Protein ID: WP_011260747.1
• Coordinates: 4761209..4761490 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRM88_RS23765
• Protein ID: WP_011260746.1
• Coordinates: 4760901..4761191 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRM88_RS23725	4756180..4756653	-	474	Protein_4270	DUF4123 domain-containing protein	-
BRM88_RS23730	4756656..4757090	-	435	WP_011409747.1	PAAR domain-containing protein	-
BRM88_RS23735	4757206..4757451	-	246	WP_069960049.1	hypothetical protein	-
BRM88_RS23745	4757787..4758119	+	333	WP_011260743.1	hypothetical protein	-
BRM88_RS23755	4758569..4759963	-	1395	WP_027703652.1	nucleoside hydrolase	-
BRM88_RS23765	4760901..4761191	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRM88_RS23770	4761209..4761490	-	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRM88_RS23775	4761585..4763837	-	2253	WP_011409750.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.83 Da
• Isoelectric Point: 10.2139

>T109240 WP_011260747.1 NZ_CP031460:c4761490-4761209 [Xanthomonas oryzae pv. oryzae]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109240 NZ_CP031460:c4761490-4761209 [Xanthomonas oryzae pv. oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109240 WP_011260746.1 NZ_CP031460:c4761191-4760901 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109240 NZ_CP031460:c4761191-4760901 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA