Detailed information of TA system TA109236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4711359..4711948
• Replicon: chromosome
• Accession: NZ_CP031459
• Organism: Xanthomonas oryzae pv. oryzae strain JL33

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: BRM84_RS23430
• Protein ID: WP_011260747.1
• Coordinates: 4711667..4711948 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRM84_RS23425
• Protein ID: WP_011260746.1
• Coordinates: 4711359..4711649 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRM84_RS23385	4706638..4707111	-	474	Protein_4208	DUF4123 domain-containing protein	-
BRM84_RS23390	4707114..4707548	-	435	WP_011409747.1	PAAR domain-containing protein	-
BRM84_RS23395	4707664..4707909	-	246	WP_069960049.1	hypothetical protein	-
BRM84_RS23405	4708245..4708577	+	333	WP_011260743.1	hypothetical protein	-
BRM84_RS23415	4709027..4710421	-	1395	WP_027703652.1	nucleoside hydrolase	-
BRM84_RS23425	4711359..4711649	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRM84_RS23430	4711667..4711948	-	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRM84_RS23435	4712043..4714295	-	2253	WP_011409750.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.83 Da
• Isoelectric Point: 10.2139

>T109236 WP_011260747.1 NZ_CP031459:c4711948-4711667 [Xanthomonas oryzae pv. oryzae]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109236 NZ_CP031459:c4711948-4711667 [Xanthomonas oryzae pv. oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109236 WP_011260746.1 NZ_CP031459:c4711649-4711359 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109236 NZ_CP031459:c4711649-4711359 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA