Detailed information of TA system TA109229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 186964..187553
• Replicon: chromosome
• Accession: NZ_CP031458
• Organism: Xanthomonas oryzae pv. oryzae strain JL28

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A8E2YMU3
• Locus tag: BRM78_RS00895
• Protein ID: WP_011260747.1
• Coordinates: 186964..187245 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRM78_RS00900
• Protein ID: WP_011260746.1
• Coordinates: 187263..187553 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRM78_RS00890	184617..186869	+	2253	WP_011409750.1	exodeoxyribonuclease V subunit alpha	-
BRM78_RS00895	186964..187245	+	282	WP_011260747.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRM78_RS00900	187263..187553	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRM78_RS00910	188491..189885	+	1395	WP_027703652.1	nucleoside hydrolase	-
BRM78_RS00920	190335..190667	-	333	WP_011260743.1	hypothetical protein	-
BRM78_RS00930	191003..191248	+	246	WP_069960049.1	hypothetical protein	-
BRM78_RS00935	191364..191798	+	435	WP_011409747.1	PAAR domain-containing protein	-
BRM78_RS00940	191801..192274	+	474	Protein_171	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.83 Da
• Isoelectric Point: 10.2139

>T109229 WP_011260747.1 NZ_CP031458:186964-187245 [Xanthomonas oryzae pv. oryzae]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQQRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109229 NZ_CP031458:186964-187245 [Xanthomonas oryzae pv. oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109229 WP_011260746.1 NZ_CP031458:187263-187553 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109229 NZ_CP031458:187263-187553 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA