Detailed information of TA system TA109224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4729328..4729917
• Replicon: chromosome
• Accession: NZ_CP031456
• Organism: Xanthomonas oryzae pv. oryzae strain HuN37

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: BRM60_RS23480
• Protein ID: WP_012443754.1
• Coordinates: 4729636..4729917 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRM60_RS23475
• Protein ID: WP_011260746.1
• Coordinates: 4729328..4729618 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRM60_RS23435	4724616..4725089	-	474	Protein_4230	DUF4123 domain-containing protein	-
BRM60_RS23440	4725092..4725526	-	435	WP_011409747.1	PAAR domain-containing protein	-
BRM60_RS23445	4725642..4725887	-	246	WP_069960049.1	hypothetical protein	-
BRM60_RS23455	4726223..4726555	+	333	WP_011260743.1	hypothetical protein	-
BRM60_RS23465	4727005..4728399	-	1395	WP_027703652.1	nucleoside hydrolase	-
BRM60_RS23475	4729328..4729618	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BRM60_RS23480	4729636..4729917	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRM60_RS23485	4730012..4732264	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T109224 WP_012443754.1 NZ_CP031456:c4729917-4729636 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T109224 NZ_CP031456:c4729917-4729636 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT109224 WP_011260746.1 NZ_CP031456:c4729618-4729328 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT109224 NZ_CP031456:c4729618-4729328 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5