Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1260155..1260747 Replicon chromosome
Accession NZ_CP031450
Organism Pseudomonas fluorescens strain SIK_W1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag DXV65_RS05720 Protein ID WP_120447727.1
Coordinates 1260155..1260433 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag DXV65_RS05725 Protein ID WP_120447728.1
Coordinates 1260442..1260747 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXV65_RS05695 1255791..1256372 + 582 WP_120447725.1 hypothetical protein -
DXV65_RS05700 1256344..1257108 - 765 WP_106805031.1 response regulator transcription factor -
DXV65_RS05705 1257202..1258023 + 822 WP_120447726.1 carbon-nitrogen hydrolase family protein -
DXV65_RS05710 1258291..1259616 + 1326 WP_065906826.1 APC family permease -
DXV65_RS05715 1259717..1259989 + 273 WP_025858669.1 type B 50S ribosomal protein L31 -
DXV65_RS05720 1260155..1260433 + 279 WP_120447727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DXV65_RS05725 1260442..1260747 + 306 WP_120447728.1 HigA family addiction module antidote protein Antitoxin
DXV65_RS05730 1260925..1262262 + 1338 WP_120447729.1 response regulator -
DXV65_RS05735 1262257..1262757 - 501 WP_120447730.1 RidA family protein -
DXV65_RS05740 1262810..1263409 - 600 WP_120447731.1 helix-turn-helix transcriptional regulator -
DXV65_RS05745 1263576..1263989 + 414 WP_120447732.1 NUDIX domain-containing protein -
DXV65_RS05750 1264004..1264921 - 918 WP_065906818.1 transcriptional regulator MetR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(13-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10518.96 Da        Isoelectric Point: 7.8618

>T109207 WP_120447727.1 NZ_CP031450:1260155-1260433 [Pseudomonas fluorescens]
MIRSFKCADTRTVFETGRSRRWSAILSVVERKLSMLDAAVELADLRSPPGNRLEPLYGDREGQHSIRINGQFRVCFVWGP
NGPENVEIVDYH

Download         Length: 279 bp

>T109207 NZ_CP031450:1260155-1260433 [Pseudomonas fluorescens]
ATGATAAGAAGCTTTAAATGCGCTGACACCAGGACCGTTTTTGAGACCGGTCGATCCAGGCGCTGGTCCGCCATCCTGTC
GGTGGTGGAACGAAAGCTGAGCATGCTCGATGCAGCGGTAGAACTCGCTGACTTGCGCTCCCCACCTGGGAACAGGCTGG
AACCCTTGTATGGCGACCGAGAGGGGCAACACAGTATCCGAATCAATGGTCAATTCCGGGTCTGTTTCGTTTGGGGCCCT
AACGGTCCGGAAAACGTTGAAATTGTGGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11011.78 Da        Isoelectric Point: 6.7170

>AT109207 WP_120447728.1 NZ_CP031450:1260442-1260747 [Pseudomonas fluorescens]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSISADVALRLAAALETSAQFWLNLQTAYDLR
TAEIAKGDQIAKEVEKVAHCA

Download         Length: 306 bp

>AT109207 NZ_CP031450:1260442-1260747 [Pseudomonas fluorescens]
ATGTTTAAGAACGGCATGCGACCTGTGCACCCTGGAGAAATTCTCAAGGAGGAGTACTTGGAGCCGCTGGGCCTGACGGC
CGCCGCCCTTGCAAGGGCGCTGCACGTTTCTGCGCCGACGGTGAATGACATTGTGTTGATGCGTCGCAGCATCAGTGCCG
ATGTTGCATTACGCCTGGCAGCTGCGTTGGAAACGTCCGCGCAGTTCTGGCTGAACCTGCAGACGGCCTATGATCTGCGC
ACAGCGGAAATCGCCAAGGGCGACCAGATAGCCAAAGAGGTCGAGAAGGTTGCGCATTGTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References