Detailed information of TA system TA109205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5863953..5864548
• Replicon: chromosome
• Accession: NZ_CP031449
• Organism: Pseudomonas aeruginosa strain 97

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CP913_RS28510
• Protein ID: WP_003117425.1
• Coordinates: 5864270..5864548 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CP913_RS28505
• Protein ID: WP_003113527.1
• Coordinates: 5863953..5864258 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP913_RS28470	5859092..5859940	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CP913_RS28480	5860107..5861048	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CP913_RS28485	5861165..5861779	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CP913_RS28490	5861821..5862405	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CP913_RS28495	5862446..5863546	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CP913_RS28505	5863953..5864258	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CP913_RS28510	5864270..5864548	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP913_RS28520	5864877..5867105	+	2229	WP_003113525.1	TonB-dependent receptor	-
CP913_RS28525	5867175..5867822	-	648	WP_003095021.1	carbonate dehydratase	-
CP913_RS28530	5867884..5869122	-	1239	WP_003162396.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T109205 WP_003117425.1 NZ_CP031449:c5864548-5864270 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T109205 NZ_CP031449:c5864548-5864270 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT109205 WP_003113527.1 NZ_CP031449:c5864258-5863953 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT109205 NZ_CP031449:c5864258-5863953 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5