Detailed information of TA system TA109184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4741599..4742169
• Replicon: chromosome
• Accession: NZ_CP031418
• Organism: Nocardia farcinica strain W6977

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DXT66_RS21805
• Protein ID: WP_060594483.1
• Coordinates: 4741599..4741880 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXT66_RS21810
• Protein ID: WP_060594484.1
• Coordinates: 4741867..4742169 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXT66_RS31245	4736793..4737011	+	219	WP_060594476.1	SRPBCC domain-containing protein	-
DXT66_RS31250	4737016..4737252	+	237	WP_060594477.1	SRPBCC domain-containing protein	-
DXT66_RS21775	4737252..4737605	+	354	WP_060594478.1	nuclear transport factor 2 family protein	-
DXT66_RS21780	4737895..4738581	-	687	WP_060594479.1	hypothetical protein	-
DXT66_RS21785	4738574..4739179	-	606	WP_060594480.1	TetR/AcrR family transcriptional regulator	-
DXT66_RS21795	4739572..4740330	+	759	WP_139337549.1	hypothetical protein	-
DXT66_RS21800	4740320..4741441	+	1122	WP_060594482.1	XRE family transcriptional regulator	-
DXT66_RS21805	4741599..4741880	+	282	WP_060594483.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DXT66_RS21810	4741867..4742169	+	303	WP_060594484.1	HigA family addiction module antidote protein	Antitoxin
DXT66_RS21815	4742433..4743215	+	783	WP_060595137.1	DUF4111 domain-containing protein	-
DXT66_RS21820	4743246..4743455	+	210	WP_107103140.1	antibiotic biosynthesis monooxygenase	-
DXT66_RS21825	4743585..4744169	-	585	WP_060594485.1	hypothetical protein	-
DXT66_RS21830	4744166..4744693	-	528	WP_159005545.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
DXT66_RS21835	4744837..4746027	-	1191	Protein_4348	MFS transporter	-
DXT66_RS21840	4746109..4746579	+	471	WP_041560401.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4736413..4755356	18943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10984.48 Da
• Isoelectric Point: 10.5558

>T109184 WP_060594483.1 NZ_CP031418:4741599-4741880 [Nocardia farcinica]
VIRSFGDKETERLWRRERVRSLDPRIHRVALRKLRQVGSAGSLEDLRIPPGNRLEALKGDRVGQHSIRINDQWRICFRWT
DAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T109184 NZ_CP031418:4741599-4741880 [Nocardia farcinica]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAGCGGGTGCGGTCACTCGATCCGAGGATCCA
TAGGGTTGCACTTCGCAAACTTCGTCAGGTGGGATCCGCTGGGTCTCTTGAGGACCTTCGCATCCCGCCTGGCAACCGAC
TCGAAGCACTCAAGGGTGATCGGGTGGGGCAGCACAGCATTAGGATCAACGACCAGTGGCGGATCTGTTTCCGATGGACG
GACGCCGGACCGGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10874.43 Da
• Isoelectric Point: 5.0747

>AT109184 WP_060594484.1 NZ_CP031418:4741867-4742169 [Nocardia farcinica]
MTTTDKIAPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGVTADTALRLAKYFGTSAEFWLNLQSHYEL
DRAEDIAGEQIAAIVPLGAA

Nucleotide

• Length: 303 bp

>AT109184 NZ_CP031418:4741867-4742169 [Nocardia farcinica]
TTGACTACCACTGACAAGATCGCGCCGATTCACCCCGGAGAAGTCCTCATGGAGGATTTCATCGAGGGCTTCGGGATCAC
GCAGAACAAACTCGCCGTGTCGATCGGAGTGCCACCTCGGCGGATCAACGAGATCGTGCACGGCAAGCGGGGGGTCACCG
CAGACACCGCGCTGCGGCTTGCGAAGTACTTCGGTACCTCGGCGGAGTTCTGGCTCAACCTGCAGAGTCACTACGAGCTG
GATCGTGCTGAGGACATCGCTGGGGAACAGATTGCTGCAATCGTTCCACTGGGTGCTGCGTGA