Detailed information of TA system TA109164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22238..22896
• Replicon: plasmid pACICU2
• Accession: NZ_CP031382
• Organism: Acinetobacter baumannii ACICU

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DMO12_RS19120
• Protein ID: WP_000312250.1
• Coordinates: 22537..22896 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DMO12_RS19115
• Protein ID: WP_001096429.1
• Coordinates: 22238..22537 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMO12_RS19070	17262..18542	+	1281	WP_001093570.1	hypothetical protein	-
DMO12_RS19075	18647..18904	+	258	WP_000834290.1	hypothetical protein	-
DMO12_RS19080	18909..19481	+	573	WP_000443897.1	hypothetical protein	-
DMO12_RS19085	19457..19636	+	180	WP_000387630.1	hypothetical protein	-
DMO12_RS19090	19653..20282	+	630	WP_000701003.1	hypothetical protein	-
DMO12_RS19095	20322..20540	+	219	WP_001043201.1	hypothetical protein	-
DMO12_RS19100	20560..21114	+	555	WP_000790084.1	hypothetical protein	-
DMO12_RS19105	21164..21700	+	537	WP_000731978.1	hypothetical protein	-
DMO12_RS19115	22238..22537	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
DMO12_RS19120	22537..22896	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DMO12_RS19125	23097..23663	+	567	WP_000710385.1	hypothetical protein	-
DMO12_RS19130	23712..23894	+	183	WP_000373385.1	hypothetical protein	-
DMO12_RS19135	23961..24659	+	699	WP_000873188.1	hypothetical protein	-
DMO12_RS19140	24798..25181	+	384	WP_000654348.1	hypothetical protein	-
DMO12_RS19145	25248..26006	+	759	WP_001053127.1	hypothetical protein	-
DMO12_RS19155	27062..27376	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa	-	1..70101	70101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T109164 WP_000312250.1 NZ_CP031382:c22896-22537 [Acinetobacter baumannii ACICU]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T109164 NZ_CP031382:c22896-22537 [Acinetobacter baumannii ACICU]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT109164 WP_001096429.1 NZ_CP031382:c22537-22238 [Acinetobacter baumannii ACICU]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT109164 NZ_CP031382:c22537-22238 [Acinetobacter baumannii ACICU]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA