Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2038144..2038646 Replicon chromosome
Accession NZ_CP031355
Organism Escherichia coli O145 strain RM8995-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DXE49_RS10610 Protein ID WP_000767829.1
Coordinates 2038144..2038398 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DXE49_RS10615 Protein ID WP_001259255.1
Coordinates 2038395..2038646 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE49_RS10580 2033159..2033386 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DXE49_RS10585 2033400..2034458 - 1059 WP_000492332.1 transport protein YeeE -
DXE49_RS10590 2034637..2035995 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DXE49_RS10595 2035985..2036047 - 63 WP_010723108.1 membrane protein YoeI -
DXE49_RS10600 2036262..2037191 - 930 WP_000803351.1 LysR family transcriptional regulator -
DXE49_RS10605 2037237..2038061 - 825 WP_000754755.1 SDR family oxidoreductase -
DXE49_RS10610 2038144..2038398 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DXE49_RS10615 2038395..2038646 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DXE49_RS10620 2038930..2038980 + 51 WP_001364200.1 his operon leader peptide -
DXE49_RS10625 2039126..2040025 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DXE49_RS10630 2040031..2041335 + 1305 WP_001400693.1 histidinol dehydrogenase -
DXE49_RS10635 2041332..2042402 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
DXE49_RS10640 2042402..2043469 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109130 WP_000767829.1 NZ_CP031355:c2038398-2038144 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109130 NZ_CP031355:c2038398-2038144 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109130 WP_001259255.1 NZ_CP031355:c2038646-2038395 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109130 NZ_CP031355:c2038646-2038395 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References