Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2163144..2163646 Replicon chromosome
Accession NZ_CP031353
Organism Escherichia coli O145 strain RM9154-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DXE50_RS12325 Protein ID WP_000767829.1
Coordinates 2163392..2163646 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DXE50_RS12320 Protein ID WP_001259255.1
Coordinates 2163144..2163395 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE50_RS12295 2158321..2159388 - 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DXE50_RS12300 2159388..2160458 - 1071 WP_001099213.1 histidinol-phosphate transaminase -
DXE50_RS12305 2160455..2161759 - 1305 WP_001400693.1 histidinol dehydrogenase -
DXE50_RS12310 2161765..2162664 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
DXE50_RS12315 2162810..2162860 - 51 WP_001364200.1 his operon leader peptide -
DXE50_RS12320 2163144..2163395 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DXE50_RS12325 2163392..2163646 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DXE50_RS12330 2163729..2164553 + 825 WP_000754755.1 SDR family oxidoreductase -
DXE50_RS12335 2164599..2165528 + 930 WP_096846611.1 LysR family transcriptional regulator -
DXE50_RS12340 2165743..2165805 + 63 WP_010723108.1 membrane protein YoeI -
DXE50_RS12345 2165795..2167153 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DXE50_RS12350 2167332..2168390 + 1059 WP_000492332.1 transport protein YeeE -
DXE50_RS12355 2168404..2168631 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T109087 WP_000767829.1 NZ_CP031353:2163392-2163646 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T109087 NZ_CP031353:2163392-2163646 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT109087 WP_001259255.1 NZ_CP031353:2163144-2163395 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT109087 NZ_CP031353:2163144-2163395 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References