Detailed information of TA system TA109053

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1725099..1725470
• Replicon: chromosome
• Accession: NZ_CP031349
• Organism: Escherichia coli O145 strain RM9467-C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: DXE51_RS08825
• Protein ID: WP_021500490.1
• Coordinates: 1725276..1725470 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1725099..1725277 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE51_RS08800	1721053..1722426	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
DXE51_RS08805	1722555..1723490	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DXE51_RS08810	1723543..1724778	-	1236	WP_000040838.1	site-specific integrase	-
DXE51_RS08815	1724780..1724995	-	216	WP_000079604.1	excisionase XisR	-
DXE51_RS08820	1725095..1725283	-	189	WP_001298826.1	DUF1187 family protein	-
-	1725099..1725277	+	179	NuclAT_0	-	Antitoxin
-	1725099..1725277	+	179	NuclAT_0	-	Antitoxin
-	1725099..1725277	+	179	NuclAT_0	-	Antitoxin
-	1725099..1725277	+	179	NuclAT_0	-	Antitoxin
DXE51_RS08825	1725276..1725470	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DXE51_RS08830	1725534..1726586	-	1053	WP_001004423.1	recombinase RecT	-
DXE51_RS08835	1726598..1729723	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
DXE51_RS08840	1729825..1730100	-	276	WP_001519830.1	phage protein	-
DXE51_RS08845	1730175..1730351	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1713391..1798062	84671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T109053 WP_021500490.1 NZ_CP031349:c1725470-1725276 [Escherichia coli O145]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T109053 NZ_CP031349:c1725470-1725276 [Escherichia coli O145]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT109053 NZ_CP031349:1725099-1725277 [Escherichia coli O145]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin