Detailed information of TA system TA109024

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1725074..1725445
• Replicon: chromosome
• Accession: NZ_CP031347
• Organism: Escherichia coli O145 strain RM9873-C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: DXE52_RS08825
• Protein ID: WP_021500490.1
• Coordinates: 1725251..1725445 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1725074..1725252 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE52_RS08800	1721028..1722401	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
DXE52_RS08805	1722530..1723465	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DXE52_RS08810	1723518..1724753	-	1236	WP_000040838.1	site-specific integrase	-
DXE52_RS08815	1724755..1724970	-	216	WP_000079604.1	excisionase XisR	-
DXE52_RS08820	1725070..1725258	-	189	WP_001298826.1	DUF1187 family protein	-
-	1725074..1725252	+	179	NuclAT_0	-	Antitoxin
-	1725074..1725252	+	179	NuclAT_0	-	Antitoxin
-	1725074..1725252	+	179	NuclAT_0	-	Antitoxin
-	1725074..1725252	+	179	NuclAT_0	-	Antitoxin
DXE52_RS08825	1725251..1725445	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DXE52_RS08830	1725509..1726561	-	1053	WP_001004423.1	recombinase RecT	-
DXE52_RS08835	1726573..1729698	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
DXE52_RS08840	1729800..1730075	-	276	WP_001519830.1	phage protein	-
DXE52_RS08845	1730150..1730326	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1716258..1788735	72477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T109024 WP_021500490.1 NZ_CP031347:c1725445-1725251 [Escherichia coli O145]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T109024 NZ_CP031347:c1725445-1725251 [Escherichia coli O145]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT109024 NZ_CP031347:1725074-1725252 [Escherichia coli O145]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin