Detailed information of TA system TA109010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3480748..3481475
• Replicon: chromosome
• Accession: NZ_CP031345
• Organism: Escherichia coli O145 strain RM12367-C1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: DXE53_RS17855
• Protein ID: WP_000547563.1
• Coordinates: 3481164..3481475 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DXE53_RS17850
• Protein ID: WP_000126294.1
• Coordinates: 3480748..3481167 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE53_RS17830	3475797..3476324	-	528	WP_001078777.1	electron transport protein HydN	-
DXE53_RS17835	3476473..3477486	-	1014	WP_024221754.1	DNA-binding transcriptional regulator AscG	-
DXE53_RS17840	3477744..3479201	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
DXE53_RS17845	3479210..3480634	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
DXE53_RS17850	3480748..3481167	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
DXE53_RS17855	3481164..3481475	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
DXE53_RS17860	3481637..3482392	-	756	WP_001400787.1	hypothetical protein	-
DXE53_RS17865	3482435..3482902	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
DXE53_RS17870	3482895..3483305	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
DXE53_RS17875	3483302..3484069	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
DXE53_RS17880	3484069..3484611	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
DXE53_RS17885	3484621..3486330	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T109010 WP_000547563.1 NZ_CP031345:c3481475-3481164 [Escherichia coli O145]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T109010 NZ_CP031345:c3481475-3481164 [Escherichia coli O145]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT109010 WP_000126294.1 NZ_CP031345:c3481167-3480748 [Escherichia coli O145]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT109010 NZ_CP031345:c3481167-3480748 [Escherichia coli O145]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22