Detailed information of TA system TA108995

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1820338..1820709
• Replicon: chromosome
• Accession: NZ_CP031345
• Organism: Escherichia coli O145 strain RM12367-C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: DXE53_RS09495
• Protein ID: WP_021500490.1
• Coordinates: 1820515..1820709 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1820338..1820516 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE53_RS09470	1816292..1817665	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
DXE53_RS09475	1817794..1818729	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DXE53_RS09480	1818782..1820017	-	1236	WP_000040838.1	site-specific integrase	-
DXE53_RS09485	1820019..1820234	-	216	WP_000079604.1	excisionase XisR	-
DXE53_RS09490	1820334..1820522	-	189	WP_001298826.1	DUF1187 family protein	-
-	1820338..1820516	+	179	NuclAT_0	-	Antitoxin
-	1820338..1820516	+	179	NuclAT_0	-	Antitoxin
-	1820338..1820516	+	179	NuclAT_0	-	Antitoxin
-	1820338..1820516	+	179	NuclAT_0	-	Antitoxin
DXE53_RS09495	1820515..1820709	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DXE53_RS09500	1820773..1821825	-	1053	WP_001004423.1	recombinase RecT	-
DXE53_RS09505	1821837..1824962	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
DXE53_RS09510	1825064..1825339	-	276	WP_001519830.1	phage protein	-
DXE53_RS09515	1825414..1825590	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1818782..1875783	57001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T108995 WP_021500490.1 NZ_CP031345:c1820709-1820515 [Escherichia coli O145]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T108995 NZ_CP031345:c1820709-1820515 [Escherichia coli O145]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT108995 NZ_CP031345:1820338-1820516 [Escherichia coli O145]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin