Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3879382..3880109 Replicon chromosome
Accession NZ_CP031341
Organism Escherichia coli O145 strain RM12275-C1

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag DXE55_RS19715 Protein ID WP_000550189.1
Coordinates 3879795..3880109 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag DXE55_RS19710 Protein ID WP_000560266.1
Coordinates 3879382..3879798 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE55_RS19700 3875472..3877016 + 1545 WP_000258466.1 hypothetical protein -
DXE55_RS19705 3877320..3879337 + 2018 Protein_3773 NADPH-dependent 2,4-dienoyl-CoA reductase -
DXE55_RS19710 3879382..3879798 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
DXE55_RS19715 3879795..3880109 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
DXE55_RS19720 3880394..3881530 - 1137 WP_000018698.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
DXE55_RS19725 3881615..3882118 + 504 WP_001295542.1 M48 family metallopeptidase -
DXE55_RS19730 3882195..3882887 + 693 WP_000942541.1 vancomycin high temperature exclusion protein -
DXE55_RS19735 3882966..3883952 + 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T108960 WP_000550189.1 NZ_CP031341:c3880109-3879795 [Escherichia coli O145]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T108960 NZ_CP031341:c3880109-3879795 [Escherichia coli O145]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT108960 WP_000560266.1 NZ_CP031341:c3879798-3879382 [Escherichia coli O145]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT108960 NZ_CP031341:c3879798-3879382 [Escherichia coli O145]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATTTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References