Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2630011..2630513 Replicon chromosome
Accession NZ_CP031341
Organism Escherichia coli O145 strain RM12275-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DXE55_RS13785 Protein ID WP_000767829.1
Coordinates 2630011..2630265 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DXE55_RS13790 Protein ID WP_001259255.1
Coordinates 2630262..2630513 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DXE55_RS13755 2625026..2625253 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DXE55_RS13760 2625267..2626325 - 1059 WP_000492332.1 transport protein YeeE -
DXE55_RS13765 2626504..2627862 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DXE55_RS13770 2627852..2627914 - 63 WP_010723108.1 membrane protein YoeI -
DXE55_RS13775 2628129..2629058 - 930 WP_000803351.1 LysR family transcriptional regulator -
DXE55_RS13780 2629104..2629928 - 825 WP_000754755.1 SDR family oxidoreductase -
DXE55_RS13785 2630011..2630265 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DXE55_RS13790 2630262..2630513 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DXE55_RS13795 2630797..2630847 + 51 WP_001364200.1 his operon leader peptide -
DXE55_RS13800 2630992..2631891 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DXE55_RS13805 2631897..2633201 + 1305 WP_001400693.1 histidinol dehydrogenase -
DXE55_RS13810 2633198..2634268 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
DXE55_RS13815 2634268..2635335 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T108955 WP_000767829.1 NZ_CP031341:c2630265-2630011 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T108955 NZ_CP031341:c2630265-2630011 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT108955 WP_001259255.1 NZ_CP031341:c2630513-2630262 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT108955 NZ_CP031341:c2630513-2630262 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References