Detailed information of TA system TA108941

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1774000..1774371
• Replicon: chromosome
• Accession: NZ_CP031341
• Organism: Escherichia coli O145 strain RM12275-C1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: DXE55_RS09225
• Protein ID: WP_021500490.1
• Coordinates: 1774177..1774371 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1774000..1774178 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DXE55_RS09200	1769954..1771327	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
DXE55_RS09205	1771456..1772391	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DXE55_RS09210	1772444..1773679	-	1236	WP_000040838.1	site-specific integrase	-
DXE55_RS09215	1773681..1773896	-	216	WP_000079604.1	excisionase XisR	-
DXE55_RS09220	1773996..1774184	-	189	WP_001298826.1	DUF1187 family protein	-
-	1774000..1774178	+	179	NuclAT_0	-	Antitoxin
-	1774000..1774178	+	179	NuclAT_0	-	Antitoxin
-	1774000..1774178	+	179	NuclAT_0	-	Antitoxin
-	1774000..1774178	+	179	NuclAT_0	-	Antitoxin
DXE55_RS09225	1774177..1774371	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DXE55_RS09230	1774435..1775487	-	1053	WP_001004423.1	recombinase RecT	-
DXE55_RS09235	1775499..1777550	-	2052	Protein_1722	PD-(D/E)XK nuclease-like domain-containing protein	-
DXE55_RS09240	1777604..1778816	+	1213	Protein_1723	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1765184..1822090	56906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T108941 WP_021500490.1 NZ_CP031341:c1774371-1774177 [Escherichia coli O145]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T108941 NZ_CP031341:c1774371-1774177 [Escherichia coli O145]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT108941 NZ_CP031341:1774000-1774178 [Escherichia coli O145]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin