Detailed information of TA system TA108926

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47376..47793
• Replicon: plasmid pEsST2350_SE_2
• Accession: NZ_CP031323
• Organism: Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: DW289_RS24985
• Protein ID: WP_001302184.1
• Coordinates: 47635..47793 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	47376..47570 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DW289_RS24950	42619..42810	+	192	WP_001027500.1	hypothetical protein	-
DW289_RS24960	43311..43838	+	528	WP_162821103.1	single-stranded DNA-binding protein	-
DW289_RS24965	43896..44129	+	234	WP_000005990.1	DUF905 family protein	-
DW289_RS24970	44195..46159	+	1965	WP_115431757.1	ParB/RepB/Spo0J family partition protein	-
DW289_RS24975	46214..46648	+	435	WP_115431758.1	conjugation system SOS inhibitor PsiB	-
DW289_RS24980	46645..47364	+	720	WP_089596794.1	plasmid SOS inhibition protein A	-
-	47376..47570	+	195	NuclAT_0	-	Antitoxin
-	47376..47570	+	195	NuclAT_0	-	Antitoxin
-	47376..47570	+	195	NuclAT_0	-	Antitoxin
-	47376..47570	+	195	NuclAT_0	-	Antitoxin
DW289_RS24985	47635..47793	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
DW289_RS24995	48340..48627	+	288	WP_001523521.1	hypothetical protein	-
DW289_RS25680	48746..49566	+	821	Protein_53	DUF932 domain-containing protein	-
DW289_RS25005	49862..50452	-	591	WP_167778863.1	transglycosylase SLT domain-containing protein	-
DW289_RS25015	50798..51181	+	384	WP_000124983.1	relaxosome protein TraM	-
DW289_RS25020	51371..52057	+	687	WP_000332492.1	PAS domain-containing protein	-
DW289_RS25025	52162..52389	+	228	WP_115431759.1	conjugal transfer relaxosome protein TraY	-
DW289_RS25030	52423..52788	+	366	WP_115431760.1	type IV conjugative transfer system pilin TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	paa / papH / papC / papD	1..82461	82461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T108926 WP_001302184.1 NZ_CP031323:47635-47793 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T108926 NZ_CP031323:47635-47793 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT108926 NZ_CP031323:47376-47570 [Escherichia coli]
TCACACGGATTACCCGTAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin