Detailed information of TA system TA108910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2110182..2110784
• Replicon: chromosome
• Accession: NZ_CP031321
• Organism: Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DW289_RS10780
• Protein ID: WP_000897305.1
• Coordinates: 2110182..2110493 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DW289_RS10785
• Protein ID: WP_000356397.1
• Coordinates: 2110494..2110784 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DW289_RS10750	2105212..2105997	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
DW289_RS10755	2106096..2106695	+	600	WP_001315111.1	glucose-1-phosphatase	-
DW289_RS10760	2106689..2107561	+	873	WP_000920762.1	virulence factor BrkB family protein	-
DW289_RS10765	2107558..2107995	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DW289_RS10770	2108040..2108981	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DW289_RS10775	2109045..2109953	-	909	WP_001162704.1	alpha/beta hydrolase	-
DW289_RS10780	2110182..2110493	+	312	WP_000897305.1	hypothetical protein	Toxin
DW289_RS10785	2110494..2110784	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DW289_RS10795	2111389..2111607	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DW289_RS10800	2111826..2112068	+	243	WP_001086388.1	hypothetical protein	-
DW289_RS10805	2112398..2113327	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DW289_RS10810	2113324..2113959	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DW289_RS10815	2113956..2114858	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T108910 WP_000897305.1 NZ_CP031321:2110182-2110493 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T108910 NZ_CP031321:2110182-2110493 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT108910 WP_000356397.1 NZ_CP031321:2110494-2110784 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT108910 NZ_CP031321:2110494-2110784 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7