Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 64723..65225 Replicon chromosome
Accession NZ_CP031321
Organism Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DW289_RS00370 Protein ID WP_000767829.1
Coordinates 64723..64977 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DW289_RS00375 Protein ID WP_001259255.1
Coordinates 64974..65225 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DW289_RS00340 59738..59965 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DW289_RS00345 59979..61036 - 1058 Protein_57 transport protein YeeE -
DW289_RS00350 61215..62573 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DW289_RS00355 62563..62625 - 63 WP_010723108.1 membrane protein YoeI -
DW289_RS00360 62840..63770 - 931 Protein_60 LysR family transcriptional regulator -
DW289_RS00365 63816..64640 - 825 WP_000754749.1 SDR family oxidoreductase -
DW289_RS00370 64723..64977 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DW289_RS00375 64974..65225 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DW289_RS00380 65508..65558 + 51 WP_001364200.1 his operon leader peptide -
DW289_RS00390 65704..66603 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DW289_RS00395 66609..67913 + 1305 WP_001349967.1 histidinol dehydrogenase -
DW289_RS00400 67910..68980 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
DW289_RS00405 68980..70047 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T108903 WP_000767829.1 NZ_CP031321:c64977-64723 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T108903 NZ_CP031321:c64977-64723 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT108903 WP_001259255.1 NZ_CP031321:c65225-64974 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT108903 NZ_CP031321:c65225-64974 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References