Detailed information of TA system TA108873

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 192613..192883
• Replicon: plasmid pGD31-F1928
• Accession: NZ_CP031295
• Organism: Escherichia coli strain EC17GD31

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DWB25_RS25895
• Protein ID: WP_001312861.1
• Coordinates: 192725..192883 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	192613..192676 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB25_RS25855	187838..188809	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
DWB25_RS25870	190189..190728	+	540	WP_032332913.1	single-stranded DNA-binding protein	-
DWB25_RS25875	190785..191018	+	234	WP_000005987.1	DUF905 family protein	-
DWB25_RS25880	191046..191243	+	198	Protein_218	hypothetical protein	-
DWB25_RS25885	191298..191732	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DWB25_RS25890	191729..192445	+	717	WP_127070542.1	plasmid SOS inhibition protein A	-
DWB25_RS27930	192457..192645	-	189	WP_001299721.1	hypothetical protein	-
-	192457..192681	+	225	NuclAT_1	-	-
-	192457..192681	+	225	NuclAT_1	-	-
-	192457..192681	+	225	NuclAT_1	-	-
-	192457..192681	+	225	NuclAT_1	-	-
-	192613..192676	-	64	-	-	Antitoxin
DWB25_RS25895	192725..192883	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
DWB25_RS25910	193804..194091	+	288	WP_000107535.1	hypothetical protein	-
DWB25_RS25915	194209..195030	+	822	WP_001234426.1	DUF945 domain-containing protein	-
DWB25_RS25920	195327..195929	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
DWB25_RS25925	196250..196633	+	384	WP_001151524.1	relaxosome protein TraM	-
DWB25_RS25930	196820..197509	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / tet(A) / aph(3')-Ia / sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA	1..245305	245305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T108873 WP_001312861.1 NZ_CP031295:192725-192883 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T108873 NZ_CP031295:192725-192883 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT108873 NZ_CP031295:c192676-192613 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin