Detailed information of TA system TA108868

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 126765..127019
• Replicon: plasmid pGD31-F1928
• Accession: NZ_CP031295
• Organism: Escherichia coli strain EC17GD31

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DWB25_RS25470
• Protein ID: WP_001351576.1
• Coordinates: 126813..127019 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	126765..126826 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWB25_RS25445	123513..124259	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
DWB25_RS25450	124318..125178	+	861	WP_000704513.1	alpha/beta hydrolase	-
DWB25_RS25455	125281..125841	+	561	WP_000139315.1	fertility inhibition protein FinO	-
DWB25_RS25460	125977..126189	+	213	WP_001299730.1	ANR family transcriptional regulator	-
DWB25_RS28105	126435..126509	+	75	Protein_144	endonuclease	-
-	126765..126826	-	62	NuclAT_3	-	Antitoxin
-	126765..126826	-	62	NuclAT_3	-	Antitoxin
-	126765..126826	-	62	NuclAT_3	-	Antitoxin
-	126765..126826	-	62	NuclAT_3	-	Antitoxin
DWB25_RS25470	126813..127019	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
DWB25_RS25475	127303..127560	+	258	WP_000083845.1	replication regulatory protein RepA	-
DWB25_RS25485	127792..127866	+	75	WP_001442103.1	RepA leader peptide Tap	-
DWB25_RS25490	127859..128341	+	483	WP_001273588.1	hypothetical protein	-
DWB25_RS25495	128334..129191	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
DWB25_RS27925	129892..130032	+	141	WP_001333237.1	hypothetical protein	-
DWB25_RS25505	130130..130780	+	651	WP_127070541.1	CPBP family intramembrane metalloprotease	-
DWB25_RS25510	130873..131130	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DWB25_RS25515	131063..131464	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / tet(A) / aph(3')-Ia / sitABCD / blaTEM-1B	iucA / iucB / iucC / iucD / iutA	1..245305	245305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T108868 WP_001351576.1 NZ_CP031295:126813-127019 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T108868 NZ_CP031295:126813-127019 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT108868 NZ_CP031295:c126826-126765 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin